去卵巢大鼠子宫内膜阴离子转运性质的变化  

Changes of the Anion Transport in the Endometria of the Ovariectomized Rats

作　　者：付小锁[1] 李利生[1] 郭华[1] 侯晓莉[1] 徐敬东[1] 

机构地区：[1]首都医科大学基础医学院生理系,北京100069

出　　处：《生殖与避孕》2008年第12期705-709,713,共6页Reproduction and Contraception

基　　金：国家自然科学基金(30770799;30740053);北京市自然科学基金(7062031;70763070);高等学校博士学科专项科研基金(20050025001);首都医科大学基础-临床基金(2006JL05和2007JL56)

摘　　要：目的:卵巢激素对子宫内膜离子转运的影响及其可能机制。方法:采用短路电流(Isc)技术,体外测量动情期大鼠(正常对照组)及去卵巢大鼠(模型组)子宫内膜电流及电阻的变化。结果:模型组的基础Isc及跨膜电阻(R)分别为25.56±7.58μA/cm2和64.57±5.44?/cm2,均高于对照组(8.91±3.18μA/cm2;48.48±4.66?/cm2,P<0.05)。使用上皮Na+通道阻断剂阿米洛利对基础电流无明显影响;cAMP依赖的Cl-通道(CFTR)激动剂弗斯可林(10μmol/L)使Isc略有增加;CFTR阻断剂格列本脲使对照组的基础电流略有降低(P>0.05),而模型组则大幅度降低(P<0.05)。Ca2+激活的Cl-通道(CACC)阻断剂DIDS可使各组的Isc均降低,组内用药前后比较,P均<0.05;而Na+-K+-2Cl-共转运体的阻断剂布美他尼(100mmol/L)对二组Isc无明显影响。结论:去卵巢大鼠的子宫内膜的CFTR和CACC对阻断剂的敏感性均强于对照组,说明上皮离子的转运与卵巢分泌的雌、孕激素有关。Objective： To investigate the property of the endometrium ion transport in the normal estruation and ovariectomize rat. Methods： The basic electrophysiological property of the endometrium was carried out by mean of the short circuit current （Isc） recording with the model rats. Results： The baseline Isc and the transepithelial resistance （R） were 8.91±3.18 μAcm^2 （n=13） and 48.48±4.66 Ω/cm^2 （n=16） in the control, 25.56±7.58 μA/cm^2 （P〈0.05, n=12） and 64.57 ±5.44 Ω/cm^2 （P〈0.05, n=15） in the ovariectomized group. Apical application of Na^＋ channel blocker, amiloride （10 μmol/L）, did not significantly affect the Isc in the two groups. Application of the endometrial epithelium with the CPTR Cl^- channel agonist, forskolin （10 μmol/L）, did not significantly increase the Isc in the two groups. Moreover, the Isc in the model group was more sensitive to the CFTR Cl^- channel blocker （glibenclamide, 1 mmol/L, apical side） than that in the control. Apical application CACC Cl^- channel blocker, DIDS （4,4＇- diisothiocyanostilbene-2, 2＇-disulfonic, 100 μmol/L）, the Isc in the model group was much more sensitive than that in the control. However, basolateral application of bumetanide （100 μmol/L, basolateral）, a blocker of Na^＋-K^＋-2Cl^- cotransport, didn＇t significantly reduce the Isc in the two groups. Conclusion： The Isc baseline and membrane resistance in the model group is much higher than that in the control, and the endometrial epithelium Cl^- secretion may be mediated by predominantly calcium-dependent Cl^- channels （CACC） and activating adenylate cyclase and apical cAMP-dependent Cl^- channels （CFTR） in the model group with minor contributions in the control. Furthermore, the anion transport in endometrial epithelium is related to estrogen and progestin which secreted by ovary.

关 键 词：子宫内膜 短路电流(Isc) 去卵巢大鼠 

分 类 号：R339.2[医药卫生—人体生理学]