奶牛GHR基因CRS-PCR多态性及其与产奶性状的关联分析  

CRS-PCR Polymorphism of GHR Gene in Chinese Holstein Cows and Its Relationship with Milk Production Traits

作　　者：王丽娟[1,2] 李秋玲[1] 王长法[1] 王洪梅[1] 李建斌[1] 高运东[1] 侯明海[1] 仲跻峰[1] 

机构地区：[1]山东省农业科学院奶牛研究中心,济南250100 [2]山东大学公共卫生学院,济南250012

出　　处：《农业生物技术学报》2008年第6期936-940,共5页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)(No.2007AA10Z169);山东省良种工程项目(No.2007LZ10-06);山东省农业科学院科研创新基金项目(No.2006YCX028和No.2007YCX019);山东省农业科学院青年科研基金项目(No.2006YQN038)资助

摘　　要：采用PCR-RFLP技术对中国荷斯坦牛生长激素受体(GHR)基因第8外显子进行单核苷酸多态性分析。结果表明,GHR基因经TasⅠ酶切后产生2种等位基因和3种基因型。等位基因A和T的频率分别为0.6339和0.3661。基因型AA、AT和TT的频率分别为0.4558、0.350和0.191。测序分析表明TT型和AA型相比在GHR基因第4962位碱基处发生T→A突变,该突变导致氨基酸由苯丙氨酸(TTT)变为酪氨酸(TAT)。χ2适合性检验表明,GHR基因的TasⅠ酶切位点处于Hardy-Weinberg不平衡状态(P<0.05)。最小二乘分析表明:基因型TT所对应的乳脂率最小二乘均值显著高于基因型AA所对应的最小二乘均值(P<0.05)。Single nucleotide polymorphisms （SNPs） of exon 8 of growth hormone receptor （GHR） gene were detected in Chinese Holstein cows by PCR-RFLP. The results showed that the exon 8 of GHR gene digested by Tas Ⅰ could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and T were 0.6339 and 0.3661 respectively. The frequencies of genotype AA, AT and TT were 0.458, 0.350 and 0.191, respectively. Sequencing revealed that one single nucleotide mutation T→A at 4 962 bp in genotype TT compared with genotype AA resulted in an amino acid change ofphenylalanine （TTT）→tyrosine（TAT）. The result of Chi Square test indicated that the frequencies of pattern types of GHR gene digested by Tas Ⅰ were not fit with Hardy-Weinberg equilibrium in this population （P 〈 0.05）. The least squares means of fat percentage in genotype TT were higher than that in genotype AA （P〈 0.05）.

关 键 词：中国荷斯坦牛 生长激素受体基因(GHB) 产奶性状 单核苷酸多态性 创造酶切位点PCR(CRS-PCR) 

分 类 号：S188[农业科学—农业基础科学]