BLU基因慢病毒表达载体的构建  

Construction of BLU Gene Recombinant Lentivirus Vector

作　　者：陈刚建[1] 张宝[2] 李燕[1] 朴仲贤[1] 陈松建[1] 刘俊晓[1] 黄晋荣[1] 郑桂娜[1] 郭勇勇[1] 霍霞[1] 

机构地区：[1]汕头大学医学院分析细胞学实验室,广东汕头515041 [2]南方医科大学生物化学教研室,广东广州510515

出　　处：《汕头大学医学院学报》2008年第4期193-195,F0002,共4页Journal of Shantou University Medical College

基　　金：国家自然科学基金资助项目(30600733)

摘　　要：目的:构建BLU基因慢病毒表达载体。方法:采用PCR技术从人脐带来源的间充质干细胞中扩增出BLU基因,将其接入慢病毒载体(pSL6-IRES-EGFP)中,经菌落PCR、酶切和测序鉴定,然后转染293T细胞观察表达情况。结果:PCR、酶切和测序鉴定克隆了重组子(pSL6-BLU-IRES-EGFP),并在293T细胞中成功表达BLU基因。结论:成功地克隆了含有BLU基因的慢病毒表达载体。Objective： To construct the lentivims vector containing BLU gene. Methods： The full-length BLU gene was amplified by PCR technique from the total RNA of the umbilical mesenchymal stem cells. BLU gene was subcloned into pSL6-IRES-EGFP vector. The identification was performed by analysis of restricting enzyme digestion and DNA sequence. Results： pSL6-BIu-IRES-EGFP was constructed successfully and identified by PCR, digestion and sequencing. The recombinant could express in 293T cell. Conclusion： The recombinant lentivirus vector containing BLU gene is cloned successfully.

关 键 词：BLU基因 慢病毒载体 

分 类 号：Q785[生物学—分子生物学]