苏铁蕨多糖的提取及其组分分析  

Purification and analysis of the Brainia insignis(Hook.) J.Sm.polysaccharides

作　　者：樊亚鸣[1] 陶文琴[2] 高绍中[1] 黄晓兰[3] 陈怡莎[1] 艾小红[1] 陈永亨[4] 

机构地区：[1]广州大学化学化工学院,广东广州510006 [2]广州大学生命科学学院,广东广州510006 [3]中国广州分析测试中心,广东广州510275 [4]广州大学环境科学与工程学院,广东广州510006

出　　处：《广州大学学报（自然科学版）》2008年第6期46-49,共4页Journal of Guangzhou University:Natural Science Edition

基　　金：国家星火计划项目(2005EA780029);广东省自然科学基金资助项目(06300617);广东省科技计划项目(2006B20901006)

摘　　要：采用低温-微波工艺提取苏铁蕨[Brainia insignis(Hook.)J.Sm.]的木质根状茎部分,以95%乙醇、水为溶剂分步提取,获苏铁蕨粗多糖,经纯化为精制多糖.以凝胶过滤色谱法(GFC)测定苏铁蕨多糖的数量及分子量,结果:苏铁蕨中含1种多糖,其数均分子量为31 514 g.mol-1,重均分子量为35 034 g.mol-1;多糖经酸解成单糖并衍生为乙酰化糖肟,对照乙酰化的标样采用气相色谱法(GC)测定其中含5种单糖组分,其相对含量:葡萄糖88.816%;半乳糖4.631%;阿拉伯糖3.314%;木糖2.085%;甘露糖1.154%.The upstanding part of xylem eaudex for Brainia insignis （ Hook. ） J. Sm. was respectively extracted by 95% ethanol and distilled water in lower temperature and under mild microwave irradiation, and the crude Brainia insignis polysaccharide was obtained and then purified further to the refined polysaccharide. Testing the number and molecular weight of Brainia insignis polysaccharide by Gel Filtration Chromatography（GFC）, the result is that there is only one kind of Brainia insignis polysaccharide and its Mn is 31 514 g · mol^-1 and Mw is 35 034 g · mol^-1. After the polysaccharides were hydrolyzed in acidic condition, the corresponding monosaccharides formed and were transformed into acetyl oximes by hydroxylamine and acetic anhydride. The derivatives were tested by Gas chromatography （GC） to give the result that the five monosaccharides exist with the certain proportion： glucose 88. 816%, galactose 4.631%, arabinose 3. 314%, xylose 2.085% ,and mannose 1. 154%.

关 键 词：苏铁蕨 多糖 单糖组分 

分 类 号：R281.5[医药卫生—中药学]