醋柳黄酮和银杏叶提取物含量测定的差异性  

Study on its variability of determination method of Total Flavones of total flavones of Hippophae rhamnoides and Ginko Biloba Extract

作　　者：孙华[1] 王玮[2] 凌春生[2] 周绍华[2] 李燕真[2] 

机构地区：[1]河南大学第一附属医院药剂科,河南开封475001 [2]河南大学药学院药剂教研室,河南开封475004

出　　处：《河南大学学报（医学版）》2008年第4期24-28,共5页Journal of Henan University:Medical Science

基　　金：河南省教育厅自然科学研究计划项目(2006350002)

摘　　要：目的:分别以槲皮素、异鼠李素为对照品测定醋柳黄酮与银杏叶提取物中总黄酮含量,研究是否可以用槲皮素代替异鼠李素作对照品。方法:采用分光光度法测定醋柳黄酮与银杏叶提取物中总黄酮含量。结果:以槲皮素折合,醋柳黄酮中总黄酮含量为8.90%,银杏提取物中总黄酮为4.41%;以异鼠李素折合,醋柳黄酮中总黄酮含量为16.80%,银杏提取物中总黄酮含量为8.25%。结论:分别以槲皮素、异鼠李素为对照品测得同一样品,总黄酮含量差异很大,不能简单用槲皮素代替异鼠李素进行样品总黄酮的含量测定。Objective： To determine the total flavones of Hippophae rhamnoides（TFH） and Ginko Biloba Extract （GBE）, and to study whether isorhamnetin can be replaced by quercetin with quercetin and isorhamnetin as reference materials. Methods： Quercetin and isorhamnetin were selected as reference material and Aluminum chloride spectrophotometry was used to determine the total flavones of Hippophae rhamnoides（TFH） and Ginko Biloba Extract （GBE）. Results： To convert into quercetin, the total flavones of TFH was 8.90%, the total flavones of GBE was 4.41%; To convert into isorhamnetin, the total flavones of TFH was 16.8%, the total flavones of GBE was 8.25%. Conclusion： The total flavones of the same sample is quite different by using different reference materials. As reference material, isorhamnetin can not be simply taken place by quercetin.

关 键 词：分光光度法 醋柳黄酮 银杏提取物 

分 类 号：R927.2[医药卫生—药学]