与人遗传病相关的三核苷酸(GAA)_n·(CTT)_n重复序列的分子克隆  

Molecular cloning on(GAA)_n·(CTT)_n repeat sequences associated with human genetic disease

作　　者：姜志艳[1] 蔡禄[1] 赵秀娟[1] 

机构地区：[1]内蒙古科技大学生物与化学工程学院,内蒙古包头014010

出　　处：《内蒙古科技大学学报》2008年第3期260-263,共4页Journal of Inner Mongolia University of Science and Technology

基　　金：国家自然科学基金资助项目(30460038);内蒙古自然科学基金重点资助项目(200508010102)

摘　　要：为了获得长片段(GAA)_n·(CTT)_n重复序列进而对其结构特性和致病机理研究,通过化学合成重复序列及两侧接头,两侧接头有Eco R I,Bam H I和Ple I酶切位点,Eco R I,Bam H I位点用于将重复序列插入pUC19制成的载体,Ple I酶切位点可方便地将重复序列从重组质粒中取出.为核苷酸重复序列的动力学结构和探讨与遗传病相关的重复序列结构特性及该类遗传疾病的分子机理奠定了基础.To clone （GAA）n·（CTT）n repeats for the further investigation on structural feature and mechanism with repeats sequence causing diseases.There exist EcoR I,BamH I and Ple I sites in the chemically synthesized repeat sequences and adaptors. The site of EcoR I and Bam H I were used to insert repeats sequences into vector made from pUC19,while Ple I sites can be used to take out the repeat sequences from recombinant plasmid easily. This work laid the foundation for the study of dynamic structure of nucleotide repeat sequences, and for the further investigation on structural properties of repeat sequences and molecular mechanism of genetic diseases.

关 键 词：人遗传病 GAA 重复序列 分子克隆 

分 类 号：Q524[生物学—生物化学]