2007-2008年泰安市流行期流行性感冒病毒分离及分析  

Isolation and analysis of influenza virus during 2007-2008 epidemic season in Tai'an city,Shandong province

作　　者：陆娟娟[1] 刘运强[1] 侯佩强[1] 

机构地区：[1]山东省泰安市疾病预防控制中心,山东泰安271000

出　　处：《疾病监测》2008年第11期680-681,共2页Disease Surveillance

摘　　要：目的对山东省泰安市2007-2008年流行期流行性感冒(流感)病毒进行分离鉴定及分析,了解泰安市流感流行情况。方法采集流感样病例(ILI)的咽拭子标本用犬肾传代细胞(MDCK)进行病毒分离,采用血凝抑制方法(HAI)进行流感病毒型别鉴定。结果2007年10月至2008年3月共检测2所哨点医院ILI咽拭子标本281份,分离到流感病毒94株,阳性分离率为33.45%,经分型鉴定A型H3N2亚型51株,H1N1亚型1株;B型的Yamagata系39株,Victoria系3株。结论2007-2008流行季中,泰安市流感病毒流行的优势毒株为A型H3N2亚型和B型Ymagata系,同时有A型H1N1亚型和B型Victoria系毒株的存在。Objective To isolate the influenza viruses and analyze the influenza activity in 2007 to 2008 epidemic season in Tai＇ an city, Shandong province. Methods Influenza viruses were isolated from the throat swabs collected from the influenza-like cases by Madin-Darby canine kidney （MDCK） cells and identified by hemagglutination inhibition （HAI） assay. Results From October, 2007 to March, 2008, a total of 281 throat swab samples from 2 surveillance hospitals were tested, and 94 strains of influenza viruses were isolated with the positive rate of 33.45%. By subtyping, 51 strains of A （H3N2） virus, 1 strain of A （H1N1） virus, 39 strains of B/Yamagata lineage virus, and 3 strains of B/Victoria lineage virus were detected. Conclusion During 2007 to 2008 influenza season, the predominant strains were A （H3N2） and B/Yamagata lineage viruses in Tai＇an, and A （H1N1） and B/Victoria lineage viruses were also in circulation.

关 键 词：流感样病例 流感病毒分离 MDCK细胞 

分 类 号：R511.7[医药卫生—内科学]