三明地区长柄石杉中石杉碱甲的提取和测定  

Ultrasonic extraction and determination of Huperzine A from H.Javanica in Sanming region

作　　者：杨煌记[1,2] 黄晖[1,2] 陈东菊[1,2] 张君诚[1] 

机构地区：[1]三明学院应用生物技术研究所,福建三明365004 [2]三明学院化学与生物工程系,福建三明365004

出　　处：《应用化工》2008年第12期1416-1418,共3页Applied Chemical Industry

基　　金：福建省科技厅重点项目(2006F6002);福建省教育厅科技项目(JA08235);三明学院大学生创新性实验计划项目(ZL0712/CS)

摘　　要：寻找超声提取长柄石杉中石杉碱甲的最佳工艺条件和石杉碱甲含量的测定方法。通过正交实验对工艺参数进行优化,并采用HPLC法加以测定。提取最佳工艺条件为:固液比为20∶1(W/V),酸浓度为2.0%,超声时间10 min;测得两地长柄石杉中石杉碱甲的含量分别为0.0263%,0.0445%。以最佳工艺条件提取石杉碱甲并测定其含量的实验方法可行,且简便、快捷、重现性好。To study the ultrasonic extraction and determination of Huperzine A from H. Jacanica. The process for Huperzine A in H. Jacanica was optimumed by orthogonal experiment design, and the content of Huperzine A was determined by HPLC. The results showed that the optimization process were as following：the ratio of solid to solution 20 ： 1 ,tartaric acid concentration 2.0% and ultrasonic time 10 min;the content of Huperzine A from two places is 0.026 3% and 0. 044 5% respectively. The method of ultrasonic extraction and determination of Huperzine A is feasible, simple, quick, and with good reproducibility.

关 键 词：长柄石杉 石杉碱甲 超声提取 含量测定 

分 类 号：R282[医药卫生—中药学] O647.3[医药卫生—中医学]