海昆肾喜对糖尿病肾病大鼠肾脏保护作用及其对肾组织TGF-β_1蛋白及mRNA表达的影响  

Protective effects of Haikunshenxi on the kidney and its influence on the expressions of transforming growth factor-β_1 and mRNA in diabetic rats

作　　者：张新雪[1] 赵宗江[1] 李杨[1] 吴国文 杨美娟[1] 

机构地区：[1]北京中医药大学,北京100029 [2]吉林省辉南长龙生化药业股份有限公司,吉林辉南135100

出　　处：《中国海洋药物》2008年第6期26-30,共5页Chinese Journal of Marine Drugs

基　　金：教育部"长江学者和创新团队发展计划"资助项目(IRT0413);国家自然科学基金重点资助项目(No:30130220);北京市中医管理局"51510"资助项目(JJ2007-006)

摘　　要：目的探讨海昆肾喜对糖尿病肾病大鼠肾组织转化生长因子-β1(TGF-β1)蛋白及mRNA表达的影响。方法50只大鼠空腹一次性腹腔注射链脲佐菌素造成糖尿病模型,并按血糖高低随机分为2组、试验组灌胃海昆肾喜,给药12周。第4、8、12周末收集大鼠24h尿量及检测尿蛋白量。12周末处死大鼠,取血并分离血清检测肌酐(Scr)、尿素氮(BUN)和甘油三酯(TG)等,取肾后固定包埋切片,采用HE、Mallory染色观察肾组织病理改变,并应用免疫组化检测肾组织TGF-β1及用原位杂交检测肾组织TGF-β1mRNA的表达。结果海昆肾喜能够降低糖尿病大鼠的蛋白尿,增加体重,降低血清Scr和BUN,改善肾功能,调节TG,改善脂代谢紊乱,并且能够降低糖尿病大鼠肾小管上皮细胞、肾间质细胞浆TGF-β1及mRNA的表达。结论海昆肾喜可能抑制TGF-β1蛋白及mRNA的过度表达,进而减缓糖尿病肾病的病程进展。Objective To study the expression of tansforming growth factor（TGF）-β1 and mRNA in the renal tubules of Streptozotocin（STZ）- induced diabetic nephropathy rats. Methods SD rats were randomly divided into two groups： normal control and diabetic nephropathy. Diabetes was induced by intraperitoneal injection of STZ. And the diabetic rats were randomly divided into four groups： model group, irbesartan treatment group, Haikunshenxi low and high dose group. Twenty-four-hour urine volume and urinary protein excretion were examined at week 4, 8, and 12 respectively. All the rats were sacrificed at week 12, and body weight, kidney weight, and serum excretion, serum creatinine, blood urea nitrogen were examined. The expression of TGF-β1 and mRNA in rat renal tissue were detected respectively by immunohistochemistry and in situ hybridization. The graphic analysis system and SPSS 11. 5 statistics software were used to treat the results. Results The expression of TOF-β1 and mRNA in the Haikunshenxi group was depressed greatly （P〈0.01） in the renal tubular epithelial cell and renal interstitial cell. Conclusions The overexpression of TGF-β1 and mRNA in renal tissue of diabetic rats may be one of the mechanisms of diabetic nephropathy,Haikunshenxi had certain renoprotective effects.

关 键 词：糖尿病肾病 海昆肾喜 转化生长因子-Β1 

分 类 号：R965[医药卫生—药理学]