反式白藜芦醇对破骨细胞分化的影响  

Effect of Trans-resveratrol on the Differentiation of Osteoclast Induced from Bone Marrow in Mice

作　　者：裴凌鹏[1] 

机构地区：[1]中央民族大学少数民族传统医学研究院,北京100081

出　　处：《河南师范大学学报（自然科学版）》2008年第6期114-116,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(30171169)

摘　　要：目的:研究反式白藜芦醇对体外破骨细胞分化的影响.方法建立由骨保护素配体(RANKL)和巨噬细胞集落刺激因子(M-CSF)共同细胞因子的小鼠破骨细胞骨髓诱导体系,将不同浓度的反式白藜芦醇作用于破骨细胞.受试细胞分为对照组、反式白藜芦醇低剂量组(10-8mol.L-1)、反式白藜芦醇中剂量组(10-7mol.L-1)和反式白藜芦醇高剂量组(10-6mol.L-1),并设立空白对照组.7 d后取细胞玻片进行抗酒石酸酸性磷酸酶(TRAP)染色,观察破骨细胞并计数;测量抗酒石酸酸性磷酸酶(TRAP)活性以及破骨细胞表面NF-κB活化受体(RANK)mRNA表达量.结果诱导培养的破骨细胞形态特征明显;反式白藜芦醇中、高剂量组在细胞数量、TRAP活性上与对照组相比有明显统计学差异(P<0.05);反式白藜芦醇各剂量组在(RANK)mRNA表达量上与对照组相比有明显统计学差异(P<0.05),且呈量效关系.结论:反式白藜芦醇可以抑制体外培养的破骨细胞分化.Objective：Effect of trans-resveratrol on the differentiation of osteoclast induced from bone marrow in vitro. Methods Mononuclear cells of mice bone marrow are incubated with DMEM containing macrophage colony stimulating factor （M-CSF） and receptor activator of NF-κB ligand （RANKL）. The culture is treated with trans-resveratrol of different concentrations （10^-8, 10^-7, 10^- 6 mol · L^-1）. On the 7 d, the culture cells are fixed and are stained for tartate-resistant acid phossphatase （TRAP）. The formation of osteoclasts is quantified by counting the number of TRAP ＋ multinuclear cells. TRAP activity and the expression of receptor activator of NF-κB （RANK） mRNA are measured. Results： The appearance of reduced mice osteoclasts is typical. Compared with the control group, the number of TRAP＋ multinuclear cells and TRAP activity of trans-resveratrol middle-dose group and trans-resveratrol high-dose group have significantly decreased（P〈0.05）. But the expression of RANK mRNA has significantly increased（P〈0.05）being treated in different dose trans-resveratrol. Conclusion：Trans-resveratrol can inhibit osteoclasts differentiation in vitro.

关 键 词：反式白藜芦醇 破骨细胞 细胞分化 骨质疏松症 

分 类 号：Q254[生物学—细胞生物学]