RP-HPLC测定积雪苷缓释片释放液中的2个指标成分  

Simultaneous Determination of Madecassoside and Asiaticoside Released from the Centella asiatica Sustained Tablets by RP-HPLC

作　　者：尹丽娜[1] 王胜浩[1] 王虎根[1] 

机构地区：[1]浙江省医学科学院,杭州310013

出　　处：《中国现代应用药学》2008年第6期527-529,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省中医药管理局重点项目(2004KF001);浙江省经贸委中药现代化项目(2006-164-43)

摘　　要：目的建立可同时测定积雪苷缓释片释放液中的2个指标成分——积雪草苷（asiaticoside，As）和羟基积雪草苷（madecassoside，MS）的RP-HPLC：方法以DIAMONSIL C18（250mm×4．6min，5μm）为色谱柱，乙腈-甲醇-水（22：35：50）为流动相。柱温控制在25℃，流速0．8mL·min^-1，检测波长为204nm，建立了可同时用于释放液中的AS和MS分离测定的RP-HPLC。结果方法学评价结果显示，积雪草总苷中MS和AS的峰面积与浓度之间呈良好的线性（浓度范围均为2～200μg·mL^-1，r=0．9999），方法的回收率MS为99．9％-101．0％，AS为100．3％-103．1％（n=6），日内日间精密度小于1．9％。结论本方法可同时测定积雪苷缓释片释放液中的2个指标成分。方法专属性强，快速简单，重现性好。OBJECTIVE To establish an HPLC method for simultaneous determination of madecassoside and asiaticoside released from the Centella asiatica sustained tablets in vitro release. METHODS HPLC analysis was performed on a DIAMONSIL C18 column （250 mm× 4.6 mm,5 μm） with the acetonitrile-methanol-water（ 22： 35： 50） as mobile phase, the column temperature was set at 25℃ ,flow rate was 0.8 mL · min^ -1 and detecting wavelength was at 204 nm. RESULTS The linear response of madecassoside and asiaticoside both ranges from 2 to 200 μg · mL^-1（ r = 0. 999 9） , the recoveries （n = 6 ） of madecassoside and asiaticoside were 99.9% -101.0% and 100.3% - 103. 1%, respectively. The precisions for assay of asiaticoside and madecassoside were within 1.9%. CONCLUSION The method is accuate, simple and reproducible, which is suitable for the determination of madecassoside and asiaticoside of the CenteUa asiatica sustained tablets.

关 键 词：积雪苷缓释片 高效液相色谱法 积雪草苷 羟基积雪草苷 体外释放 

分 类 号：R917.101[医药卫生—药物分析学] R917.4[医药卫生—药学]