红毛五加总苷对大鼠体内疼痛介质的影响  

Effects of Glucosides of Acanthopanax Giraldii on Pained Mediators in Rats in Vivo

作　　者：许国敏 郑加嘉 黄东亮 龙启才[3] 

机构地区：[1]深圳市布吉人民医院,广东深圳518112 [2]深圳市一致药业股份有限公司,广东深圳518029 [3]中山大学药学院,广东广州510080

出　　处：《深圳中西医结合杂志》2008年第6期329-333,共5页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：广东省中医药管理局科研立项课题(1060147)

摘　　要：目的:探讨灌胃给药(ig)红毛五加总苷(TGA)对大鼠体内疼痛介质的影响。方法:用试剂盒测定大鼠igTGA后对5%甲醛(FD)致痛模型血清及脑组织的NO,NOS,β-EP和CGRP的变化。结果:大鼠igTGA200mg/kg并皮下注射FD5min后,血清和脑NO,NOS,β-EP含量与模型组比较P>0.05。大鼠igTGA200mg/kg和400mg/kg并皮下注射FD5min和60min后CGRP含量与模型组比较P>0.05。大鼠igTGA400mg/kg并注射FD5min后,NO(123.29±27.28)mol/L血清,(1.86±0.17)mol/gprot脑;NOS(15.03±3.16)U/L血,(1.55±0.19)U/mgprot脑;β-EP(108.23清±6.43)ng/L血清,(139.48±15.32)ng/g脑,各组与模型组比较P<0.01。大鼠igTGA200mg/kg并注射FD60min后,NO(141.27±24.12)mol/L血清,(1.84±0.30)mol/gprot脑;NOS(17.35±2.77)U/L血清,(1.51±0.23)U/mgprot脑;β-EP(111.48±11.72)ng/L血清,(132.48±19.46)ng/g脑;大鼠igTGA400mg/kg并注射FD60min后,NO(129.20±30.39)mol/L血清,(1.65±0.31)mol/gprot脑;NOS(14.23±2.94)U/L血清,(1.29±0.30)U/mgprot脑;β-EP(121.80±12.25)ng/L血清,(152.78±13.08)ng/g脑;各组与模型组比较P<0.01。结论:大鼠igTGA200mg/kg和400mg/kg主要影响注射FD引起的Ⅱ相疼痛应激反应(60min),对Ⅰ相疼痛应激反应(5min)的作用较轻。推测TGA的镇痛作用可能不影响体内CGRP,但与NO,NOS,β-EP变化有关。Objective To explore effects of ig Glucosides of Acanthopanax Giraldii （TGA） on pained mediators in rats in vivo. Methods Changes of NO, NOS, β-EPand CGRP in rat＇s serum and brain tissues were determined by kits following ig TGA and subcutaneous injection （sc） of 5% formaldehyde （FD）. Results Contents of NO, NOS, β-EP in rat＇s serum and brain were no significant difference （P 〉 0.05; vs modle control） after ig 200 mg/kg TGA and sc FD 5 min later. CGRP also did not change （P 〉 0.05; vs modle control） after ig 200 mg/kg or 400 mg/kg TGA and sc FD 5 min and 60 min. NO （123.29 ± 27.28） μmol/L （1.86±0.17）μmo1/gprot train; NOS （15.03±3.16） U/L （1.55±0.19） U/mgprot brain; β-EP （108.23±6.43）ng/L （139.48±15.32） ng/gbnin,in were obviously alteration （P 〈 0.01; vs modle control） when ig 400 mg/kg TGA and 5 min after sc FD to rats. NO （141.27±24.12）μmol/L （1.84±0.30）μmol/gprototrais; NOS （17.35±2.77） U/Lstrum, （1.51±0.23） U/mgprOt train;β-EP （111.48±11.72） ng/L （132.48±19.46） ng/gbrain were markedly variation （P 〈 0.01; vs modle control） while ig 200 mg/kg TGA and 60 min after sc FD to rats. NO （129.20±30.39）μmol/L （1.65±0.31）μmol/gprot train; NOS （14.23±2.94） U/L （1.29±0.30） U/mgprot train; β-EP （121.80±12.25） ng/L （152.78±13.08） ng/g brain were also changes （P 〈 0.01; vs modle control） following ig 400 mg/kg TGA and 60 min after sc FD to rats. Conclusion Rats ig 200 mg/kg and 400 mg/kg TGA mainly influence phase Ⅱ pained stress reaction （60 min） induced by sc FD, whereas mildly influence phase Ⅰ pained stress reaction （5 min）. We speculated that analgesic effects of TGA might not alter CGRP, but be related to changes of NO, NOS and β- EP in vivo.

关 键 词：红毛五加总苷 N0 NOS Β-EP CGRP 

分 类 号：R285[医药卫生—中药学]