Effects of N-methyl berbamine on delayed outward potassium current in isolated rat hepatocytes  

作　　者：李金鸣[1,2] 崔桂英[1,2] 刘东举[1,2] 崔宏[1,2] 常天辉[1,2] 王玉萍[1,2] 张克义[1,2] 

机构地区：[1]中国医科大学药理教研室 [2]中国医科大学第一临床学院普外一科

出　　处：《中国药理学报》1998年第1期24-26,共3页Acta Pharmacologica Sinica

摘　　要：目的：研究Ｎ甲基小檗胺（ＮＭＢ）对大鼠肝细胞外向钾电流的影响．方法：应用膜片箝技术和全细胞记录方法，箝制电位－５０ｍＶ，指令电位＋３０至＋１４０ｍＶ，持续时间９００ｍｓ．结果：ＮＭＢ以浓度依赖方式降低外向钾电流．电流幅值在２０，５０，４００ｎｍｏｌ·Ｌ－１和５０μｍｏｌ·Ｌ－１时分别从４４±１０（ｎ＝４），２５±１８（ｎ＝４），５８±２１（ｎ＝５），４６±１３（ｎ＝６）ｎＡ降到３６±０４（Ｐ＞００５），２１±１６（Ｐ＞００５），３７±１６（Ｐ＜００５），２３±１３（Ｐ＜００１）ｎＡ．抑制率分别为１０％，１５％，３７％，５１％．结论：ＮＭＢ是一种钾通道阻断剂．AIM: To study the efftcts of N-methyl berbamine (NMB) on the delayed outward potassium currents ( I k) in isolated rat hepatocytes. METHODS: With patch clamp techniques and whole cell recording method, holding potential -50 mV , command potential +30 to +140 mV , duration 900 ms. RESULTS: NMB reducded I k in a concentration dependent manner. When the concentrations of NMB were 20, 50, 400 nmol·L -1 and 50 μmol·L -1 , the amplitude values of I k were decreased to 3 6±0 4 ( P >0 05), 2 1±1 6 ( P >0 05), 3 7±1 6 ( P <0 05), 2 3±1 3 nA ( P <0 01) from 4 4±1 0 ( n =4 ), 2 5±1 8 ( n =4), 5 8±2 1 ( n =5), 4 6±1 3 ( n =6) nA of control, respectively. The inhibitory rates were 10 %, 15 %, 37 %, and 51 %, respectively. CONCLUSION: NMB was a K + channel inhibitor.

关 键 词：甲基小檗胺 膜片箝技术 离子通道 钾 

分 类 号：R972.2[医药卫生—药品]