红豆杉愈伤组织中sinenxans高产细胞系的选择及其培养  

Selection and culture of high-yield sinenxans cell lines of Taxus spp. distributed in China

作　　者：吴蕴祺 朱蔚华[1] 陆俭[1] 胡秋[1] 李新兰[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所

出　　处：《中国药学杂志》1998年第1期15-18,共4页Chinese Pharmaceutical Journal

摘　　要：目的：对国产红豆杉愈伤组织中的ｓｉｎｅｎｘａｎｓ高产细胞系进行选择及培养，为半合成类紫杉醇抗癌药提供充足的前体化合物。方法：红豆杉培养细胞采用平板培养及二分法继代培养，应用高效液相色谱法测定培养物中的ｓｉｎｅｎｘａｎｓ含量，选择出ｓｉｎｅｎｘａｎｓ高产细胞系并扩大培养。结果：Ｓｉｎｅｎｘａｎｓ的含量在不同种红豆杉的愈伤组织及同种不同无性系间存在明显的差异，并与培养物的色泽有一定的相关性；不同的培养基组成与培养方式对高产细胞系的生长有较大影响；获得了一个ｓｉｎｅｎｘａｎｓ高产细胞系Ｔｓ１９，其ｓｉｎｅｎｘａｎｓ含量达细胞干重的４．０％；在一个培养周期中，Ｔｓ１９组织培养物生长缓慢，ｓｉｎｅｎｘａｎｓ的含量在培养前期有所下降，而在培养后期可达到最高水平。结论：在一定的培养条件下，选择出的ｓｉｎｅｎｘａｎｓ高产细胞系的ｓｉｎｅｎｘａｎｓ含量稳定。OBJECTIVE: To select and culture highyield sinenxans cell lines of Taxus spp. distributed in China to provide enough precursors for semisynthesis of taxollike anticancer drugs. METHODS: Cell cultures of Taxus were plate cultured and bisected subcultured. HPLC was used to determine the contents of sinenxans in callus cultures for selecting a highyield sinenxans cell line, and the selected highyield sinenxans cell line was cultured on a large scale. RESULTS: The contents of sinenxans were very different among not only the species, but also the clones of one species, and there was a relationship between the content and the color of the callus cultures: The growth of sinenxans highyield cell lines was affected by medium composition and culture modes; a highyield cell line, Ts19, obtained from T. chinensis produced up to 4.0% of sinenxans on dry weight basis; Ts19 callus grew slowly during the culture, and the content of sinenxans in it decreased during the early culture but reached the highest level at the end of culture. CONCLUSION: The content of sinenxans of Ts19 was relatively stable on the present culture condition.

关 键 词：红豆杉 sinenxans 高产细胞系 

分 类 号：S567.190.3[农业科学—中草药栽培]