E-选择素不同结构域缺失对其粘附功能的影响  

Study on structurefunction relationship of Eselectin

作　　者：蔡铀庆[1] 胡美茹[1] 汲言山[1] 于鸣[1] 孙瑛勋[1] 王建安[1] 沈倍奋[1] 

机构地区：[1]军事医学科学院基础医学研究所

出　　处：《中华微生物学和免疫学杂志》1998年第1期16-20,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家"863"青年基金

摘　　要：目的研究Ｅ－选择素不同结构域缺失对其粘附功能的影响。方法从人胎脐静脉内皮细胞总ＲＮＡ中用ＲＴ－ＰＣＲ方法获其全长ｃＤＮＡ，设计并合成三对缺失Ｅ－选择素分子的ＥＧＦ结构域及ＥＧＦ＋ＣＲ１＋ＣＲ２结构域引物，并将三者基因分别在痘苗病毒真核表达系统中表达。结果细胞粘附功能试验结果表明，Ｅ－选择素分子ＥＧＦ结构域或ＥＧＦ＋ＣＲ１＋ＣＲ２结构域的缺失削弱了Ｅ－选择素分子的粘附能力。此外，本研究尚克隆并在痘苗表达系统中表达了可溶性Ｅ－选择素分子。结论Ｅ－选择素分子不同结构域缺失对其粘附能力削弱现象的发现为临床某些疾病的发病机理揭示及治疗提供了线索。可溶性Ｅ－选择素分子在痘苗病毒表达系统中表达成功为与其介导的白细胞粘附。In order to research the structurefunction relationship of Eselectin,Eselectin cDNA was obtained by RTPCR from total RNAs of human fetal umbilical vein endothelial cells.Three pairs of primers were designed and synthesized to delete EGF domain and EGF+CR1+CR2 domain; Eselectin cDNA and two mutant cDNAs were expressed in TK-143 cells.U937 cell adhesion experiment showed that deletion of EGF domain or EGF+CR1+CR2 domain weakened the cell adhesion mediated by Eselectin molecules.Theses findings provided the clue for shedding light on mechanism of some diseases and their treatments.Additionally,soluble Eselectin cDNA was cloned and expressed in vaccinia virus system,which laid the basis for regulating the processes of white blood cell adhesion and tumor metastasis etc,mediated by Eselection.

关 键 词：E-选择素 粘附 痘苗病毒 结构域缺失 

分 类 号：R392.7[医药卫生—免疫学]