胶束动电毛细管色谱法测定晶福眼药水  

Determination of Vitaphakol Eye Drops by Micellar Electrokinetic Capillary Chromatography

作　　者：徐志利[1] 邓兆勇[1] 杨仲元[1] 

机构地区：[1]广州市药品检验所

出　　处：《药物分析杂志》1998年第1期12-15,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：建立了胶束动电毛细管色谱法分离和测定晶福眼药水中烟酰胺、腺苷和硫柳汞含量的测定方法．以十二烷基磺酸钠（７５ｍｍｏｌ／Ｌ）为胶束相，运行液为２０ｍｍｏｌ／Ｌ硼酸缓冲液（ｐＨ＝９．２），恒定电压２０ｋＶ，检测波长２６０ｎｍ；６ｍｉｎ内三组分达到基线分离．选用Ｌ－色氨酸作内标物进行测定，各组分线性范围：烟酰胺５～１０００ｍｇ／Ｌ（ｒ＝０．９９９６），腺苷５～３００ｍｇ／Ｌ（ｒ＝０．９９９９），硫柳汞２～２５０ｍｇ／Ｌ（ｒ＝１．００００）；平均回收率±ＲＳＤ：烟酰胺（９８．４±０．８）％，腺苷（１０１．０±１．１）％，硫柳汞（１００．４±０．３）％，ｎ＝３．此法简便快速，重现性好．A micellar electrokinetic capillary chromatographic(MECC)method for the separation and determination of niacinamide,adenosine,and thimerosal in vitaphakol eye drops was established. The complete separation of three components was achieved in 6 minutes with 75m mol/L sodium dodecyl sulfate (SDS) solution as micellar phase,20m mol/L pH92 running boric acid buffer,a constant voltage of 20kV and 260nm as detecting wavelength.Using Ltryptophan as internal standard,the linear range was 5～1000mg/L(r=09996)for niacinamide,5～300mg/L(r=09999)for adenosine,and 2～250mg/L(r=10000)for thimerosal.The recovery(±RSD)was (984±08)% for niacinamide,(1010±11)% for adenosine and (1004±03)% for thimerosal.This method is simple,rapid and reproducible.

关 键 词：胶束动电 毛细管色谱 晶福眼药水 烟酰胺 腺苷 

分 类 号：R927.2[医药卫生—药学] R988.1