海豚链球菌疫苗对罗非鱼免疫功能的影响  

Effect of Streptococcus iniae vaccine on immune response of tilapia Oreochromis niloticus

作　　者：徐增辉[1] 陈汉忠[1] 陈明[2] 李莉萍[2] 李超[1] 甘西[2] 梁万文[2] 李晓栩[1] 张居作[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西水产研究所,广西南宁530021

出　　处：《大连水产学院学报》2008年第6期413-418,共6页Journal of Dalian Fisheries University

基　　金：广西科学研究与技术开发计划基金项目(0639036);广西研究生教育创新计划项目(2007105930906M90)

摘　　要：以海豚链球菌Streptococcus iniae灭活菌苗为免疫原,通过注射、浸泡、口服3种途径对体重为(100±10)g的奥尼罗非鱼Oreochromis niloticus进行免疫,在免疫前和免疫后的第3、7、10、14、21天对试验鱼进行尾静脉采血,测定其血液中白细胞的数量、各类白细胞的数量及其吞噬活性,以及血清的抗菌活力、溶菌酶活性、超氧化物歧化酶(SOD)活力及抗体水平。在免疫后第22天对所有试验鱼按照1.0×107cfu/尾进行攻毒。结果表明:各免疫组白细胞总数、淋巴细胞、嗜中性粒细胞和单核细胞数量均显著高于对照组(P<0.05);血清的抗菌活力、溶菌酶活性和抗体水平与对照组差异显著(P<0.05),SOD活力和对照组无显著差异(P>0.05);免疫的罗非鱼对海豚链球菌的抵抗力明显增强,其中注射组罗非鱼的免疫能力明显高于浸泡和口服组,加强免疫组的免疫能力均有明显增强趋势。Healthy tilapia Oreochromis niloticus averaging（ 100 ± 10）g in body weight were vaccinated by killed Streptococcus iniae cells as immunogen via oral administration, immersion, and intraperitoneal injection. The white blood cell count （WBC）, differential leucocyte count （DLC）, phagocytes activities, bacteriolytic activities, lysozyme activities, superoxide dismutase （SOD） activities and antibody activity （OD450 nm ） were determined in the blood collected from the caudal veins of three fish in each group randomly sampled 0,3,7,10,14, and 21 days after immunization. The test fish were challenged by intraperitioneal injection （IP） （ 1.0 × 10^7 colony - forming units （cfu）/fish） of S. iniae 22 days after immunization. The tilapia immunized with vaccine showed significantly higher WBC, and the amount of lymphocyte, neutrophilic granulocyte and monocyte than those in the control group （P 〈 0.05 ）. There were significantly higher serum bacteriolytic activities, lysozyme activities and antibody activity （ OD450 nm） in the vaccinated fish than those in the fish in the control group （ P 〈 0.05 ） while the immunized fish showed no significant differences in the superoxide dismutase （SOD） activities compared with the control group（P 〉 0.05）. The higher relative percent survival （RPS） was found in the fish immunized by IP and immersion, and the highest immune ability was observed in the tilapia treated by IP.

关 键 词：罗非鱼 海豚链球菌 疫苗 免疫力 

分 类 号：S942.5[农业科学—水产养殖]