荧光法测定补骨脂素和异补骨脂素  

Determination of Psoralen and Isopsoralen by Fluorometry

作　　者：祁彦[1] 庞志功[1] 汪宝琪[1] 

机构地区：[1]西安医科大学药学院

出　　处：《分析科学学报》1998年第1期65-67,共3页Journal of Analytical Science

摘　　要：以溴代十六烷基三甲胺（ＣＴＭＡＢ）为胶束试剂，β－环糊精（β－ＣＤ）为包含试剂，在优化实验条件下，用荧光光度法测定了补骨脂中补骨脂素和异补骨脂素．线性范围分别为２×１０－９～８×１０－６ｇ／ｍＬ，２×１０－９～８×１０－７ｇ／ｍＬ；检出限分别为２．６７×１０－１１ｇ／ｍＬ和１．１５×１０－１１ｇ／ｍＬ．The content of psoralen and isopsoralen was determinated by 930 fluorospectrophotometer, which CTMAB as micellar reagent, β CD as inclusion reagent. The linear ranges of psoralen and isopsoralen are 2×10 -9 ￣8×10 -6 g/mL, 2×10 -9 ￣8×10 -7 g/mL, and their detection limit are 2.67×10 -11 g/mL, 1.15×10 -11 g/mL,respectively.

关 键 词：补骨脂素 异补骨脂素 荧光法 CTMAB Β-CD 

分 类 号：R284.1[医药卫生—中药学]