高血压大鼠肾脏中HMGCS2表达及抗氧化作用  

Different renal HMGCS2 expressions between spontaneously hypertensive rat (SHR) strains and its antioxidative role

作　　者：衣卫杰[1] 应晨江[1] 范之良[1] 孟依[1] 刘健[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030

出　　处：《中国公共卫生》2009年第1期14-16,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30471461)

摘　　要：目的探讨自发性高血压大鼠(SHR)和卒中易感型自发性高血压大鼠(SHRSP)肾脏组织中3-羟基-3-甲基戊二酰辅酶A合成酶2(HMGCS2)的表达差异及其在抗氧化中的作用。方法随机选取2月龄雄性SHR和SHRSP大鼠各50只,用免疫印迹法检测肾组织中HMGCS2和过氧化氢酶表达差异以及细胞信号磷酸化水平;同时测量组织中丙二醛(MDA)含量。结果SHRSP组HMGCS2表达量(6 398±658.128)明显低于SHR组(16516.67±1321.855)(P<0.05)。SHRSP组MDA含量[(0.738±0.175)U/mg prot]高于SHR组[(0.330±0.032)U/mg prot](P<0.05);SHRSP组蛋白激酶B(Akt)的磷酸化水平(1.909±0.124)明显高于SHR组(0.229±0.071)(P<0.05)。结论HMGCS2在SHRSP和SHR肾脏的表达差异有统计学意义,并可以在抗氧化中发挥作用;其表达可能由磷酸化蛋白激酶B(p-Akt)信号调节。Objective To compare the differet expressions of 3 - hydroxy - 3 - methylglutaryl - CoA , synthase 2 （HMGCS2） in kidney between stroke prone spontaneously hypertensive rat （SHRSP） and spontaneously hypertensive rat （SHR） strains and explore its antioxidative role. Methods Five male SHRSP and five male SHR at the age of 2 months were randomly chosen. The expressions of HMGCS2, catalase and phosphorylating signal of protein kinase B （Akt） pathway were evaluated by immunoblotting analysis. The level of malondialdehyde （MDA） was also measured, Results Lower re- nal HMGCS2 expression （6 398 ± 658. 128 vs 16516.67 ±1321. 855, P 〈 0. 05 ）, higher MDA level （0. 738 ± 0. 175 U/rag prot vs 0. 330 ±0. 032 U/mg prot,P 〈0.05） and higher Akt phosphorylation signal（ 1. 909 ±0. 124 vs 0. 229 ±0. 071 ,P 〈 0. 05） in SHRSP than those of in SHR were observed. Conclusion The different expression of renal HMGCS2 between SHRSP and SHR may be partially responsible for the antioxidative property.

关 键 词：卒中易感型自发性高血压大鼠(SHRSP) 自发性高血压大鼠(SHR) 3-羟基-3-甲基戊二酰辅酶A合成酶2 丙二醛 磷酸化蛋白激酶B 

分 类 号：R544.1[医药卫生—心血管疾病]