家族性原发性高血压与基因多态性关系  

Synergistic effects of GNB3 gene C825T and ATG gene M235T polymorphisms on familial essential hypertension

作　　者：路方红[1] 杨建民[1,2] 周晓红[1] 魏芳[3] 刘振东[1] 赵颖馨[1] 王舒健[1] 

机构地区：[1]山东省医学科学院心脑血管病防治研究中心,济南250062 [2]教育部和卫生部心血管重构和功能研究重点实验室山东大学齐鲁医院心内科 [3]山东大学附属济南市中心医院心内科

出　　处：《中国公共卫生》2009年第1期20-22,共3页Chinese Journal of Public Health

基　　金：山东省自然科学基金(y2005c25)

摘　　要：目的探讨山东省汉族人群鸟苷酸结合蛋白(G蛋白)β3亚单位基因(GNB3)C825T多态性和血管紧张素原(AGT)基因M235T多态性与家族性原发性高血压(EH)的关系。方法应用聚合酶链反应(PCR)和限制性内切酶酶切方法,对162例原发性高血压患者和180名正常对照组进行GNB3 C825T和AGT M235T多态性检测。结果EH组GNB3 C825T各等位基因频率与正常对照组比较差异无统计学意义(P>0.05);EH组AGTM235T多态性的MT+TT基因型及T等位基因频率则高于对照组,差异有统计学意义(P<0.05);在AGT TT纯合子中,GNB3 TT纯合子患病率及收缩压(SBP)、舒张压(DBP)均高于C等位基因携带者(P<0.05);逐步Logistic回归分析结果显示,825TT+235TT基因型高血压的发病危险为其他基因型的3.45倍(P=0.001),高于单基因的AGT 235TT(1.87)。结论AGT基因M235T多态性T等位基因是山东省汉族原发性高血压家系人群EH发病的危险因素之一。GNB3 825T等位基因对高血压的发病有协同作用。Objective To investigate synergistic interaction between the C825T polymorphism of G protein β3 subunit gene（ GNB3 ） and the M235T polymorphism of angiotensinogen gene（AGT） in essential hypertension（EH）. Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the C825T polymorphism of GNB3 gene and M235T polymorphism of AGT gene in 162 hypertensive patients and 180 normotensive controls. Results There was no significant difference of the GNB3 gene C825T polymorphism CC,CT,TT genotypes and T alleles between hypertensive patients and normotensive controls. The frequencies of CT ＋ TT genotypes and T allele of AGT gene M235T polymorphism in EH were significantly higher than that of in control group. The odds ratio of EH for subjects with GNB3 TT genotype and AGT TT genotype（3.45） was markedly increased compared to the subjects with AGT TT genotype（1.87） alone. Conclusion AGT 235T should be a risk factor for EH in Shandong Han population and there was a significant synergistic effect of GNB3 235T allele and AGT 235T allele in EH.

关 键 词：基因多态性 原发性高血压 协同作用 

分 类 号：R544.1[医药卫生—心血管疾病]