氧化应激在糖尿病大鼠肌病中作用  

Role of oxidative stress in experimental diabetic myopathy in rat

作　　者：王玲[1] 牛文彦 董德勇[1] 孙悦 王永明[4] 

机构地区：[1]天津医科大学生理教研室,天津300070 [2]天津医学大学免疫教研室,天津300070 [3]天津医学大学病理教研室,天津300070 [4]天津医科大学毒理教研室,天津300070

出　　处：《中国公共卫生》2009年第1期41-42,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金国际合作交流项目(30611120532)

摘　　要：目的探讨氧化应激在糖尿病肌病发生发展中的作用。方法尾静脉内注射链脲佐菌素诱导糖尿病大鼠模型。测定糖尿病不同时期股四头肌组织丙二醛含量和总超氧化物歧化酶活性。光镜下观察糖尿病不同时期股四头肌组织形态学的改变。结果糖尿病大鼠1,3和6个月股四头肌组织丙二醛水平与同期对照组相比显著增高,分别为(1.81±0.30),(0.96±0.35),P<0.01;(1.14±0.20)(0.91±0.21),P<0.05;(1.32±0.29),(0.97±0.22),P<0.05。糖尿病大鼠1个月股四头肌组织总超氧化物歧化酶活性与对照组相比明显增高(47.43±6.23),(34.21±4.04),P<0.01,3个月时降至正常对照水平,6个月时明显降低(26.10±5.89),(32.67±5.56),P<0.05。组织形态学观察表明:糖尿病大鼠3个月时股四头肌出现病理改变,随着病程延长,病理改变越严重。结论糖尿病大鼠早期股四头肌就已存在明显的氧化应激,氧化应激在糖尿病肌病的发生发展中起重要作用。Objective To explore the role of oxidative stress on the initiation and development of the diabetic myopathy of rats. Methods Diabetic rats were induced by injection of streptozotocin （STZ） into the tail vein. The malondialdehyde （MDA） level and the total superoxide dismutase（SOD） activity in the quadriceps femoris were measured at the time of 1 month,3 months and6 months of experimental diabetes. The pathological changes of diabetic rats were examined by light microscopy. Results The MDA level increased significantly in the diabetic group as compared to control group at all time intervals（1.81 ±0.30 vs0. 96±0. 35,P〈0. 01,1.14±0.20 vs 0.91 ±0.21,P〈0.05,1.32±0.29 vs 0.97±0.22 ,P〈 0.05 ）. The total SOD activity increased significantly in the diabetic group as compared to control group at 1 month （47.43 ± 6.23 vs 34.21 ± 4.04, P 〈 0. 001 ）, and dropped to baseline at 3 month, while decreased significantly at 6 month （26.10±5.89 vs 32.67±5.56 P 〈0. 05）. Inspection of the quadriceps femoris by light microscopy demonstrated that the diabetic rats exhibited pathological changes at 3 month and the changes were more serious as the diabetic duration extended. Conclusion Oxidative stress was found to occur before the initiation of diabetic myopathy and was maintained in the developing of the diabetic myopathy. This implies that the oxidative stress plays an important role in the initiation and development of the diabetic myopathy.

关 键 词：糖尿病 骨骼肌 丙二醛 超氧化物歧化酶 

分 类 号：R587.1[医药卫生—内分泌]