血管紧张素-2对内皮细胞和线粒体膜电位影响  

Effects of Ang-2 on intracellular [Ca^(2+)] i and mitochondria membrane potential in VEC

作　　者：韩磊[1] 李鸣皋[1] 马贵喜[1] 刘玉[1] 李靖[1] 蒙果[1] 刘昕[1] 

机构地区：[1]解放军海军总医院航空潜水医学中心,北京100037

出　　处：《中国公共卫生》2009年第1期50-51,共2页Chinese Journal of Public Health

基　　金：海军后勤科研基金(00-3321)

摘　　要：目的观察血管紧张-2(Ang-2)对血管内皮细胞(VEC)内[Ca2+]i及线粒体膜电位水平的影响。方法将VEC分为空白对照组和Ang-2处理组。用激光共聚焦显微镜测量各组细胞的[Ca2+]i和线粒体膜电位。结果空白对照组的细胞内[Ca2+]i为(24.781±1.350),Ang-2组的细胞内[Ca2+]i为(47.384±1.323),Ang-2组的细胞内[Ca2+]i,明显高于空白对照组(P<0.01)。空白对照组的线粒体膜电位水平为(36.578±1.1470),Ang-2组的线粒体膜电位水平为(9.016±1.124),Ang-2组的线粒体膜电位水平明显低于空白对照组(P<0.01)。结论Ang-2可引起VEC内[Ca2+]i和线粒体膜电位水平的明显改变。Objective To explore the effects of angiotensin - 2 （ Ang - 2） on intracellular free calcium concentration and mitochondria membrane potential in vascular endothelial cell（VEC）. Methods VEC were divided into control group and Ang - 2 group. Intracellular free calcium concentration and mitochondria membrane potential were measured by laser scanning confocal microscopy. Results Intracellular free calcium concentration of control group was 24. 781 ± 1.350. Intracellular free calcium concentration of Ang - 2 group was 47. 384 ± 1. 323. Intracellular free calcium concentration of Ang - 2 group was significantly higher than that of control group（P 〈0.01 ）. Mitochondria membrane potential of control group was 36. 578 ± 1. 147. Mitochondria membrane potential of Ang - 2 group was 9. 016 ± 1. 124 and significantly lower than that of control group（P 〈0. 01 ）. Conclusion Ang -2 can induce obvious changes of intracellular free calcium concentration and mitochondria membrane potential of VEC.

关 键 词：血管内皮细胞 血管紧张素-2 CA^2+ 线粒体膜电位 

分 类 号：R331.36[医药卫生—人体生理学] R852.11[医药卫生—基础医学]