4a亚型猪戊型肝炎病毒对猕猴致病性  

Study on pathogenicity of subtype 4a swine HEV in rhesus monkey

作　　者：韦献飞[1] 朱永红[2] 唐荣兰[1] 秦小云[1] 葛宪民 熊云新[1] 吉彦莉[2] 梁靖瑞[2] 谢益安[1] 

机构地区：[1]柳州医学高等专科学校公共卫生系,柳州545006 [2]北京大学医学部病原生物学系 [3]广西职业病研究所

出　　处：《中国公共卫生》2009年第1期69-70,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30570063);广西科学基金项目(桂科自:0728242)

摘　　要：目的研究4 a亚型猪戊型肝炎病毒(HEV)对猕猴的致病性。方法用1株从广西壮族自治区农村地区幼猪粪便中分离并已经基因测序确定为4 a亚型的猪HEV静脉注射感染猕猴,通过检测粪便HEV RNA、血中HEVRNA、血清丙氨酸氨基转移酶(ALT)水平、HEV抗体及肝组织分别进行病理学和电镜检查确定HEV致病性。结果猪HEV株实验感染猕猴后,出现粪便排毒、病毒血症、ALT升高及HEV抗体阳转,活检肝组织切片呈现典型的急性病毒性肝炎的病理变化,电镜下观察到肝细胞中散在有大量病毒样颗粒。结论广西4 a亚型猪HEV能够跨物种感染猕猴并引起戊型肝炎。Objective To investigate the pathogenicity of subtype 4a swine HEV in rhesus monkey. Methods Inoculate a rhesus monkey through intravenous injection with the subtype 4a swine hepatitis E virus（HEV） isolated from piglets fecal in Guangxi and identified by sequencing and evaluate its pathogenicity by detecting HEV RNA,serum ALT, anti- HEV and histopathological changes of liver biopsies. Results The infected monkeys had virus shedding,viraemia, elevated ALT and histopathological changes typical for acute virual hepatitis. In addition, virus particles were observed under electron microscope. Conclusion Subtype 4a swine HEV could experimentaUy infect rhesus monkey and induce hepatitis E.

关 键 词：戊型肝炎病毒 猪 致病性 跨物种传播 

分 类 号：R373.2[医药卫生—病原生物学]