小鼠被动吸烟引发成瘾性及对免疫指标影响  

Addiction induced by cigarettes smoke exposure and change of immunity index in mice

作　　者：杨爱红[1,2] 胡庆东[1] 李光武[1] 汪莉[1] 

机构地区：[1]安徽医科大学神经生物学与行为学研究所,合肥230032 [2]淮北职业技术学院医学系

出　　处：《中国公共卫生》2009年第1期77-79,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30470904);国家博士后科学基金(20060400201)

摘　　要：目的探讨昆明种小鼠通过被动吸烟途径引发成瘾性以及对小鼠体重和免疫力的影响。方法2月龄雄性昆明种小鼠30只,随机分为空白对照组和被动吸烟组。小鼠被动吸烟时暴露于弥漫有烟草烟雾的染毒箱中,2次/d,40 m in/次;空白对照组置于同样规格无烟雾的染毒箱中。对小鼠进行条件性位置偏爱(CPP)试验检测,体重测量,脾脏指数、胸腺指数分析,免疫比浊法检测外周血清中补体C3、C4及免疫球蛋白IgA、IgG、IgM含量。结果CPP实验显示,被动吸烟4周组与空白对照组比较差异无统计学意义(P>0.05);8周组与空白对照组及4周组比较,差异均有统计学意义(P<0.01;P<0.05)。被动吸烟小鼠体重及体重增长率明显低于空白对照组,差异均有统计学意义(P<0.01;P<0.01)。被动吸烟小鼠脾脏指数,C3、C4,IgA、IgG差异均有统计学意义(C3P<0.01;其他P<0.05);胸腺指数,IgM下降不明显(P>0.05)。结论昆明种小鼠被动吸烟可引发成瘾且伴有体重和免疫力低下。Objective To explore induced addicton of cigarettes smoke by psaaive smoking and its influence on body weight and immunity in mice. Methods Thirty 2 - month - old male Kunming mice were chosen and divided in to the control group breathing the natural air and the passive smoking group. The passive smoking group was exposed to a sealing box filled with cigarettes smoke from a stick of cigarette partly burning. The process lasting for 40 minutes was performed twice a day. Comparatively, the control group was exposed into the same size sealing box filled with natural air. The conditioned place preference （CPP） was used to test the addiction to cigarettes smoke. To test the influence of the addiction on the morphological development and immunity, the body weight, indexes of spleen and thymus gland, the content of C3, C4 and IgA, IgG, IgM in the peripheral serum were detected and analyzed, and the immunoturbidimetry （ITM） method was used. Resuits For the results of CPP, there was no statistical significance between control group and the passive smoking 4w group （ P 〉 0. 05 ） , while there were statistical significances between the control group, the passive smoking 4w group and the passive smoking 8w group （ P 〈 0.01 ; P 〈 0. 05 ）. The addiction and the withdrawal performances were observed. The body weights and the weight growth rate of the passive smoking groups were less than that of the control group （ P 〈 0.01 ）. The index of spleen,the contents of C3 ,C4 and IgA,IgG of the passive smoking groups were less than that of the control group （ all P 〈 0. 05 except C3 P 〈 0. 01 ） Conclusion The addiction could be induced by long - term cigarettes smoke exposure and the morphological development was retarded and immunity was weakened in passive smoking Kunming mice.

关 键 词：被动吸烟 成瘾性 条件性位置偏爱 免疫力 

分 类 号：R994.6[医药卫生—毒理学]