党参对间充质干细胞增殖影响  

Effects of Dangshen on proliferation of mesenchymal stem cells

作　　者：王菊光[1] 王连荣 项鹏[3] 

机构地区：[1]北京市海淀区疾病预防控制中心微生物检验科,北京100080 [2]北京市中西医结合医院骨科 [3]中山大学干细胞与组织工程研究中心

出　　处：《中国公共卫生》2009年第1期123-125,共3页Chinese Journal of Public Health

基　　金：留学回国人员科研启动基金(教外司留[2003]14号)

摘　　要：目的探讨党参（Dangshen，DS）对间充质干细胞（Mesenchymal stem cells，MSCs）增殖的影响。方法水煮醇沉法制备党参溶液，Ficoll—Paque细胞分离液（1．077×10^3g/L）分离成人MSCs，体外扩增，将P5～P10代细胞分为DS组和对照组，3d后相差显微镜观察细胞形态，四甲基偶氮噻唑蓝（MTT）微量酶反应法检测细胞代谢活性，流式细胞仪检测细胞周期和免疫表型。结果DS组细胞代谢活性（0．535±0．041），（0．972±0．129）与对照组（0．440±0．066），（0．822±0．097）相比，差异有统计学意义（P〈0．05）。DS组S期细胞百分含量（2．14±0．25）％较对照组（1．10±0．04）％增多，凋亡细胞百分含量（1．04±0．62）％较对照组（3．45±1．33）％减少，差异有统计学意义（P〈0．05）。DS组细胞CD105和CD166表达阳性，CD34和CD45表达阴性，未观察到形态学改变。结论DS能够促进MSCs的增殖，并且使MSCs仍保持高度的未分化状态。Objective To investigate the effects of Dangshen （DS） on proliferation of mesenchymal stem cells （MSCs）. Methods DS solution was distilled and precipitated in ethanol. MSCs were separated from adult human bone marrow with Ficoll -Paque reagent and expanded in culture medium. P5 - P10 cells were treated with DS （DS group） or without DS（ control group）. After 3 days ,effects of DS on cell morphology ,proliferation and immunophenotypes were analyzed with a phase microscope, MTT colorimetric assays and flow cytometry, respectively. Results Compared with the control group（ 0. 440 ± 0. 066,0. 822 ± 0. 097 ）, the metabolic activities of cells treated with DS （0. 535 ± 0. 041,0. 972 ± 0. 129） increased significantly（P 〈0. 05）. The percentage＇of S phase in DS group（2. 14 ±0.25% ） was higher than that of in control group（ 1.10 ± 0. 04% ,P 〈0. 05） and the percentage of apoptotic cells in DS group（ 1.04 ± 0. 62% ） was lower than that of in control group （3.45 ± 1.33 %, P 〈 0. 05 ）. Cells treated with DS were uniformly positive for CD105, CD166, negative for CD34, CD45 and changes in morphology were not observed. Conclusion DS can promote the proliferation of MSCs and keep the MSCs in the undifferentiated state.

关 键 词：党参 间充质干细胞 免疫表型 

分 类 号：R285[医药卫生—中药学]