pH敏感相分离高分子的免疫分析应用研究  

Preparation of pH Sensitive Phase Separating Polymers and Its Application in Immunoassay

作　　者：周平[1] 邓延倬[1] 曾云鹗[1] 

机构地区：[1]武汉大学化学系

出　　处：《高等学校化学学报》1998年第2期198-200,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：合成了溶解性受ｐＨ值影响的高分子化合物聚甲基丙烯酸甲酯－丙烯酸－顺丁烯二酸酐（ＭＡＭ）及聚甲基丙烯酸－丙烯酰胺－Ｎ－羟基琥珀酰亚胺丙烯酸酯（ＭＡＮ），分别与抗体进行了共价连接，将其用作免疫分析载体，利用其溶解性可调节特征，建立了新的免疫分析方法．以聚甲基丙烯酸－丙烯酰胺－Ｎ－羟基琥珀酰亚胺丙烯酸酯为载体，对血清中乙肝表面抗原进行了检测，阴阳性检出结果与常规ＥＬＩＳＡ方法相符．The methylmethacrylate acrylic acid maleic anhydrate copolymer(MAM) and methacrylic acid acrylamide N acryloxysuccinimide copolymer(MAN) were prepared. These copolymers were conjugated with monoclonal antibody separately and utilized in a novel separation method for enzyme immunoassay. The polymer precipitated out of water below a critical pH, enabling the polymer bound immune complex to be separated from the solution. However, the MAM antibody can show phase separation properties in urine, but can not precipitate in serum. The MAN antibody showed the insoluble form below pH 3 5 and soluble form above pH 4 0. It has been demonstrated to be applicable to determination of antigen by determining HBsAg in serum. Forty five samples were assayed, and there was a good correlation between the results by this method and ELISA.

关 键 词：溶解性 免疫分析 pH敏感相分离 乙肝表面抗原 

分 类 号：R446.6[医药卫生—诊断学]