L-四氢巴马汀对羟考酮依赖大鼠不同脑区单胺递质水平的影响  

Effect of L-tetrahydropalmatine on contents of monoamine transmitters in the brains of oxycodone-dependence rats

作　　者：刘艳丽[1,2] 颜玲娣[1] 周培岚[1] 吴春福[3] 宫泽辉[1] 

机构地区：[1]军事医学科学院毒物药物研究所新药评价实验室,北京100850 [2]苏州大学药学院,江苏苏州215123 [3]沈阳药科大学药理教研室,辽宁沈阳110016

出　　处：《中国医院药学杂志》2008年第24期2085-2088,共4页Chinese Journal of Hospital Pharmacy

基　　金：解放军总后“十五”指令性课题(编号:01L038)

摘　　要：目的:观察L-四氢巴马汀(L-THP)对羟考酮依赖大鼠不同脑区单胺递质含量的影响,探讨L-THP对抗羟考酮依赖的机制。方法:以连续递增给药建立羟考酮依赖大鼠模型,L-THP伴随给药,采用HPLC-ECD法测定大鼠前皮层、海马、纹状体和伏隔核内多巴胺(DA)和5-羟色胺(5-HT)含量的变化。结果:与空白对照组相比,羟考酮依赖的大鼠纳络酮催促戒断后前皮层内DA、高香草酸(HVA)和5-HT含量明显降低,伏隔核内DA、3,4-二羟基苯醋酸(DOPAC)、HVA和5-HT含量明显降低,海马内DA、DOPAC、5-HT含量没有明显变化,而5-吲哚乙酸(5-HIAA)明显升高,纹状体内DA及其代谢产物DOPAC、HVA以及5-HT、5-HIAA含量明显降低;L-THP(10,20,30mg·kg-1,ig)伴随给药,能不同程度抑制上述变化。结论:L-THP可抑制羟考酮依赖引起的不同脑区内单胺类递质含量的变化,提示L-THP对抗羟考酮依赖可能与调节多巴胺和5-HT系统相关。OBJECTIVE To study mechanisms of L-tetrahydropalmatine（1-THP） on oxycodone-dependence through effect of L-tetrahydropalmatine on contents of monoamine transmitter in the brain of oxycodone-dependence rats. METHODS Rats were given increasing doses of oxycodone to induce physical dependence. Withdrawal syndromes were precipitated 6 hours following the last administration of oxycodone by injection of naloxone （5 mg·kg^-1, i. p）. Contents of DA and 5-HT were measured through HPLC-ECD. RESULTS Contents of DA, HVA and 5-HT were decreased in frontal cortex, so did DA, DOPAC, HVA and 5-HT in nucleus accumbens, DA, DOPAC, HVA and 5-HT in striatum, compared with normal control. 5-HIAA was increased in hippoeampus of oxycodone-dependence rats. L-THP （10, 20, 30 mg·kg^-1 , i. g. ） could inhibit the content changes of monoamine transmitter. CONCLUSION Conclusions：L-THP inhibited changes of monoamine transmitter in brains of oxycodone-dependence rats. The results indicated that inhibited effect of L-THP on oxycodone-dependence maybe related to mediating DA and 5 HT system.

关 键 词：L-四氢巴马汀 羟考酮 DA 5-HT 

分 类 号：R963[医药卫生—微生物与生化药学]