咪唑克生对Lewis大鼠实验性自身免疫性脑脊髓炎的作用  

Intervention of idazoxan on experimental autoimmune encephalomyelitis in Lewis rats

作　　者：朱振国[1] 郑荣远[1] 李剑敏[2] 韩钊[1] 王新施[1] 陈艳艳[1] 

机构地区：[1]温州医学院附属第一医院神经内科,325000 [2]温州医学院附属第一医院病理科,325000

出　　处：《实用医学杂志》2008年第24期4183-4187,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30470611);浙江省自然科学基金资助项目(编号:Y204133)

摘　　要：目的:探讨咪唑克生(idazoxan,Ida)对Lewis大鼠实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)的干预作用,观察其对大鼠脊髓组织内小胶质细胞、星形胶质细胞的变化和血清IFN-γ、IL-10表达的影响。方法:采用豚鼠脊髓匀浆免疫诱发Lewis大鼠EAE模型,免疫当日开始给予Ida10mg/kg对其进行实验性干预,连续10d,然后对其神经症状加以评分。用免疫组化法测定腰髓中表达GFAP和CD11b的阳性细胞数;用ELISA法测定血清IFN-γ和IL-10水平。结果:与EAE模型组相比,Ida干预组大鼠的临床症状显著改善(P<0.05),而其生病率与EAE模型组差异无显著性;免疫组化结果显示,Ida干预组活化的小胶质细胞数量减少,但活化的星形胶质细胞数量增多(P<0.05)。Ida对血清中IFN-γ和IL-10的水平没有明显影响。结论:Ida对EAE大鼠具有保护作用,其作用机制可能为Ida对中枢神经系统的免疫机制有一定影响。Objective To explore the effect of idazoxan intervention on experimental autoimmune encephalomyelitis （EAE） in Lewis rats, on the changes of mieroglia and astroglia in the spinal cord, and on serum levels of IFN-γ and IL-10. Methods The murine model of EAE was established by the induction of guinea pig spinal cord homogenate and idazoxan of 10 mg/kg was administered for intervention for 10 days starting from the day of induction. The neurological symptoms were scored. The count of neurons expressing GFAP and CD11b was detected by immunohistochemistry, so was serum levels of IFN-γ and IL-10 by ELISA. Results As compared with the model group, symptoms were markedly relieved in the idazoxan group （P 〈 0.05）, but the incidence did not differ significantly. Immunohistochemical assay showed that the number of activated microglia was declined in the idazoxan group （P 〈 0.05） while that of activated astroglia was enhanced （P 〈 0.05）. Idazoxan had no effect on serum levels of IFN-γ and IL-10 （P 〉 0.05）. Conclusion Idazoxan plays a protective role in rats with EAE and its mechanism may be involved in some impact on the immunological mechanism of the CNS.

关 键 词：脑脊髓炎 自身免疫性 实验性 干扰素Ⅱ型 白细胞介素10 咪唑克生 小胶质细胞 星形胶质细胞 

分 类 号：R96[医药卫生—药理学]