当归注射液对免疫诱导再生障碍性贫血小鼠骨髓细胞CD_(34)^+及Fas抗原表达的影响  

Effect off angelicae sinensis injection on CD_(34)^+ and Fas antigen expression of bone marrow cells in immune-induced aplastic anemia mice

作　　者：李爱民[1] 吴晓珊[1] 尹忠钦[1] 罗劲松[2] 石清照[2] 徐之良[2] 万申红[1] 

机构地区：[1]华中科技大学同济医学院附属荆州医院,湖北荆州434100 [2]武汉大学人民医院

出　　处：《中国小儿血液与肿瘤杂志》2008年第6期268-270,285,共4页Journal of China Pediatric Blood and Cancer

摘　　要：目的探讨当归注射液对免疫诱导再生障碍性贫血小鼠骨髓细胞CD34+及Fas抗原表达的影响。方法建立免疫诱导再障小鼠模型,随机分为当归组、再障组、正常组,分别测定各组小鼠的体重、外周血白细胞计数、骨髓单个核细胞计数以及流式细胞仪检测骨髓单个核细胞CD34+及Fas抗原表达的荧光强度,应用SPSS10.0统计软件进行分析。结果再障组及当归组小鼠的体重、外周血白细胞计数、骨髓单个核细胞计数较正常组均有降低,但当归组明显高于再障组(P<0.05),当归组骨髓细胞CD34+抗原表达的荧光强度(69.0±8.3)明显高于再障组(33.6±9.1,P<0.05),而与正常组(78.2±9.4)之间无显著性差异(P>0.05)。再障组骨髓单个核细胞Fas抗原表达的荧光强度(62.7±7.1)明显高于正常组(21.5±3.6,P<0.05),当归组骨髓单个核细胞Fas抗原表达的荧光强度(43.0±6.8)明显低于再障组(62.7±7.1,P<0.05)。结论当归能降低再障小鼠骨髓细胞Fas抗原的表达,减少细胞凋亡,促进骨髓造血细胞增生,有刺激造血作用。Objective To investigate the effect of Angelicae Sinensis injection on CD34^＋ and Fas antigen expression of bone marrow cells in immune-induced aplastic anemia （AA） Mice. Methods The model of immune-induced AA mice were induced by means of 6.0 Gy ^60Co T-ray irradiation and lymphocyte infusion through tail vein. The mice were divided into 3 groups, the Angelicae Sinensis group, the AA group and the normal group. To measure the weight, the count of blood leucocyte and bone marrow nucleated cell, CD34^＋ antigen expression of bone marrow cells, Fas antigen expression of bone marrow cells. All dam analyzed by SPSS 10.0. Results CD34^＋ antigen expression intensity of Radix Angelicae Sinensis group was 69.0±8.3 and much higher than the AA group＇ s（33.6 ±9. 1 ,P 〈 O. 05 ）, with no statistic difference from that in normal group （ 80. 5 ± 2.6, P 〉 0. 05 ）. Fas antigen expression intensity of the AA group was 62. 7±7. 1 and much higher than the normal group＇ s（21.5 ±3.6, P 〈 0. 05 ）. Fas antigen expression intensity of Angelicae Sinensis group was 43.0 ± 6. 8 and much lower than the AA group＇ s （ 62. 7 ± 7.1, P 〈 0. 05 ）. Conclusion Angelicae Sinensis could promote proliferation of stem and progenitor cell of AA mice.

关 键 词：当归注射液 贫血 再生障碍性 小鼠 CD34^+抗原 FAS抗原 

分 类 号：R285.5[医药卫生—中药学]