腹腔神经节毁损对胃扩张兔脊髓P物质表达的影响  

Effects of celiac ganglion damage on expression of substance P in spinal cord of gastric distension rabbits

作　　者：曾令全[1] 魏安宁[2] 王智彪[1] 杜永洪[1] 李崇燕[1] 傅洪[2] 

机构地区：[1]重庆医科大学生物医学工程系,医学超声工程研究所,重庆400016 [2]重庆医科大学附属第二医院麻醉科,重庆400010

出　　处：《重庆医科大学学报》2008年第12期1414-1417,共4页Journal of Chongqing Medical University

基　　金：国家杰出青年基金(NO.30325027);教育部创新团队项目(教计函[2005]33号)资助

摘　　要：目的:观察高强度聚焦超声毁损兔腹腔神经节(Celiac ganglion,CG)对胃扩张兔脊髓P物质(Substance P,SP)表达的影响,探讨SP是否也参与内脏痛的传递。方法:按2×2析因设计,将新西兰大白兔20只,随机分为4组:非扩张非毁损组(nDnB)、扩张非毁损组(DnB)、非扩张毁损组(nDB)、扩张毁损组(DB),每组各5只。DB、nDB组用HIFU毁损神经节,nDnB、DnB组假手术,术后7d,安置胃扩张装置。安置后7d,nDB、nDnB组在麻醉下灌注固定取胸段6￣8(T6￣T8)脊髓,DB和DnB组在扩张胃1h后,灌注固定取脊髓。用免疫组织化学方法检测脊髓后角SP光密度(Optical density,OD)。结果:在后角Ⅰ、Ⅱ层内,毁损CG使中间区及其后根投射纤维内的OD增加(P<0.01),胃扩张后使内、外侧区的OD增加(P<0.01),毁损CG后,胃扩张使后角Ⅰ、Ⅱ层的内、外侧区OD增加值下降(P<0.01)。结论:胃扩张会引起胸段脊髓后角SP的表达明显增加;毁损CG使胃扩张引起的增加值下降。Objective： To observe the influence of damaging celiac ganglion（CG） by high intensity focused ultrasound （HIFU）on expression of substance P（SP） in spinal cord following gastric distension in rabbits, and study whether SP participate in transferring pain information as neurotransmitter. Mothods： 20 rabbits were randomly divided into four groups in a 2 × 2 factorial design： not distended not damaged （ nDnB ）, distended not damaged （ DnB ）, not distendedly damaged （ nDB ）, distendedly damaged（DB）,with 5 in each group. At first,group nDB and DB were damaged by HIFU,group nDnB and DnB not damaged. 7 days later,they all were installed with the device of gastric distension. In group nDB and nDnB,tissue were directly fixed through infusing 4% parafomaldehyde under deep anaesthesia,then spinal cord of thoracic 6-8 （T6～T8） was extracted. In group DB and DnB stomach was distended for 1 h,and the spinal cord were extracted in the way similar to that in the above two groups. Immunohistochemistry technique of strept avidin biotin complex（SABC ）was used to display the content of SP in spinal cord. Results： In lamina and I] of dorsal horn,damaged CG could induce the OD in afferent projections and medial zone to increas significantly（P〈0.01）. Gastric distension could induce the OD in lateral zone to increase significantly（P〈0.01）. After CG was damaged,the increase of OD in lateral zone significantly descended（P〈0.01 ）.Conclusion： Gastric distension could induce the expressionof SP in thoracic slices to increase significantly. After CG is damaged,that increase induced by gastric distension in lateral zone lamina Ⅰand Ⅱ of dorsal horn significantly descends.

关 键 词：腹腔神经节 P物质 脊髓 胃扩张 疼痛 

分 类 号：R441.1[医药卫生—诊断学]