链脲佐菌素未致高血糖大鼠腹腔葡萄糖耐量试验的动态观察  

Dynamic observation on the intraperitoneal glucose tolerance test in the non-hyperglycemic rats treated with streptozotocin

作　　者：文晓红[1,2] 沈成义[3] 雷军[4] 杨正伟[2] 王亚平[1] 

机构地区：[1]重庆医科大学基础医学院组织与胚胎学教研室,重庆400016 [2]川北医学院形态定量研究室,南充637007 [3]川北医学院病理生理研究室,南充637007 [4]川北医学院药物研究所,南充637007

出　　处：《重庆医科大学学报》2008年第12期1467-1470,共4页Journal of Chongqing Medical University

基　　金：四川省科技攻关项目(04SG022-024);四川省青年科技基金(04ZQ026-025)

摘　　要：目的:研究链脲佐菌素(Streptozotocin,STZ)未致高血糖大鼠的糖耐量有无异常。方法:10￣12周龄SD雄性大鼠随机分为2组:实验组,腹腔内注射30mg/kg(小剂量组)或60mg/kg(大剂量组)的STZ;对照组,腹腔内注射等量酸性缓冲液。注射后1￣12周对STZ未致高血糖大鼠(空腹血糖<10mmol/L)进行腹腔葡萄糖耐量试验(Intraperitoneal glucose tolerance test,IPGTT)。结果:小剂量组大鼠的血糖或IPGTT与对照组相比均无显著性差异;大剂量组未致高血糖大鼠在STZ注射后有2/9￣3/5的大鼠的IPGTT异常,即50%葡萄糖腹腔内注射(2g/kg)后120min的空腹血糖值大于相应对照组的所有动物的该值,且≥10mmol/L。结论:小剂量STZ未导致大鼠高血糖,且IPGTT无异常,而大剂量STZ未致高血糖大鼠的糖耐量减退。Objective： To study whether the glucose tolerance was impaired in the non-hyperglycemic rats treated with streptozotocin （STZ）. Methods： Male SD rats,aged 10-12 weeks,were divided into two groups：experimental group,with an intraperitoneal injection of 30 mg/kg（low dose） or 60 mg/kg（high dose） STZ;control group,with injection of equal dose acidified saline. 1-12 weeks after injection, the intraperitoneal glucose tolerance test（IPGTT） was performed in the non-hyperglycemic rats（fasting blood glucose 〈10 mmol/L） treated with STZ. Results： There was no significant difference in the fasting blood glucose or IPGTT between the control and the low dose groups. 2/9-3/5 of the non-hyperglycemic rats treated with high dose STZ had abnormal IPGTF,i.e,at 120 min after intraperitoneal injection of 50% glucose（2g/kg）, the fasting blood glucose levels were higher than those in all the animals in the corresponding control group, and≥10 mmol/L. Conclusion： Low dose STZ did not induce hyperglycemia or abnormal IPGTF in all rats,and the non-hyperglycemic rats treated with high dose STZ had an impaired glucose tolerance.

关 键 词：大鼠 糖尿病 链脲佐菌素 血糖 腹腔葡萄糖耐量试验 

分 类 号：R383.3[医药卫生—医学寄生虫学]