靶向HBV X基因的shRNA表达质粒对HBV复制的抑制作用  

HBV Replication Inhibited by shRNA Expressing Plasmid Targeting HBV X Gene

作　　者：李绍祥[1] 张成平[1] 胡大荣[1] 李娟[1] 范公忍[1] 刘超英[1] 

机构地区：[1]北京军区总医院肝病科,北京100700

出　　处：《昆明医学院学报》2008年第6期1-4,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30471541);北京军区十一五计划课题资助项目(06BJ020)

摘　　要：目的观察靶向HBV X基因的RNA干扰(RNAi)对HBV复制的抑制作用.方法选择HBV基因组X区的Nt1681-1708作为靶序列,合成相应的正、反义寡核苷酸,退火后形成双链,克隆入shRNA表达质粒,将得到的质粒与HBV质粒共转染HepG2细胞,观察HepG2细胞中HBsAg、HBeAg、HBV DNA和pgRNA的受抑制情况.结果HBsAg、HBeAg的表达、HBV DNA和pgRNA的拷贝数均明显受到抑制.结论所选靶区通过RNAi可有效抑制HBV复制.Objective To observe whether HBV replication is inhibited by RNA interference （RNAi） targeting HBV X gene. Methods Nt1681-1708 of HBV genome was selected as the target sequence, the respective sense and antisense oligonucleotide were synthesized, annealed and the double strand product was recombined into the shRNA expressing plasmid, which was then cotransfected the HepG2 cell with the HBV plasmid. Afterwards, its inhibition on HBsAg, HBeAg, HBV DNA and pgRNA were observed. Results Copies of HBV DNA, pgRNA and expression of HBsAg and HBeAg were found inhibited significantly. Conclusion The tested RNAi target region is sufficient to inhibit HBV replication.

关 键 词：RNA干扰 乙型肝炎病毒 乙型肝炎病毒表面抗原 乙型肝炎病毒E抗原 

分 类 号：R512.62[医药卫生—内科学]