生物素化葡聚糖胺示踪显示大鼠皮质脊髓束的实验研究  

Research Report of Tracing Corticospinal Tract in Rat with Biotinylated Dextran Amine

作　　者：郭西良[1] 王昭君[1] 朱兴宝[2] 冯忠堂[1] 王廷华[1] 

机构地区：[1]昆明医学院神经科学研究所,云南昆明650031 [2]成都军区昆明总医院神经外科,云南昆明650032

出　　处：《昆明医学院学报》2008年第6期25-29,共5页Journal of Kunming Medical College

基　　金：云南省自然科学基金资助项目(2004C0003R);云南省教育厅科研基金资助项目(04Z037C)

摘　　要：目的用BDA(Biotinylated Dextran Amine)示踪大鼠皮质脊髓束,观察其在脊髓内的走行.方法采用成年SD大鼠10只,分A、B两组.A组用10%BDA注射到双侧皮质运动区,B组注射到左侧皮质运动区.3周后灌注取材,取C1～3、T3～5和T9～11节段脊髓做冰冻切片,行DAB染色.结果(1)横向观察:A组在双侧脊髓白质的后索以及前正中裂两侧观察到棕色阳性纤维.B组在右侧脊髓后索和左侧前正中裂观察到棕色阳性纤维;两组都有显色纤维从后索发出进入灰质且在灰质内分布均匀;(2)纵向观察:阳性纤维自吻端向尾端逐渐稀疏.结论BDA示踪技术可以直观地显示大鼠皮质脊髓束在脊髓内走行,阳性染色纤维的数量自吻端向尾端逐渐稀疏.Objective To trace and observe corticospinal tract coursing in rat＇s spinal cord with biotinylated dextran amine （BDA）. Methods Ten adult Sprague-dawley rats were randomly divided into two groups：10 % BDA were stereotactieally injected into the both cortical motor area in group A and into left cortical motor area in group B. Three weeks later, animals were sacrificed. The spinal cord of C1-3, T3-5 and T9-11 were sliced and observed by DAB staining. Results （1） Horizontal survey： In group A, the BDA -labeled corticospinal tract fibers distributed in the whole posterior funiculus and in both banks of the anterior median fissure; In group B, the BDA - labeled fibers distributed in the right half of posterior funiculus and in the left bank of the anterior median fissure; In both groups, the BDA - labeled fibers, from posterior funiculus, entered grey matter and distributed in the whole grey matter; （2） Longitudinal survey：BDA - labeled fibers were gradually sparse from rostral side to caudal end. Conclusion BDA tracing Can clearly reveal the course of the corticospinal tract in rat＇ s spinal cord in which BDA - labeled fibers are gradually sparse from rostral side to caudal end.

关 键 词：神经纤维示踪 葡聚糖胺 皮质脊髓束 

分 类 号：Q426[生物学—神经生物学]