抑制性寡脱氧核苷酸对小鼠CD4^+ Th1细胞功能性分化的影响  

Effect of suppressive oligodeoxynucleotides on the functional differentiation in CD4^+ Th1 lymphocytes in mice in vitro

作　　者：李沙陵[1] 戴霞红[1] 范学工[1] 李宁[2] 刘洪波[1] 代洪[1] 周蓉蓉[1] 

机构地区：[1]中南大学湘雅医院传染科,长沙410008 [2]中南大学湘雅医院输血科,长沙410008

出　　处：《中南大学学报（医学版）》2008年第12期1089-1094,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30671845);教育部高校博士点基金新教师资助项目(20070533009);湖南省卫生厅科研基金资助项目(B2005030);中南大学大学生创新性实验计划项目(071053365)~~

摘　　要：目的:观察抑制性寡脱氧核苷酸对小鼠脾脏CD4+T细胞Th1分化的影响。方法:分离小鼠脾脏淋巴细胞,以免疫磁珠阳性选择法纯化CD4+T细胞,以荧光活化细胞分拣术分析其分选纯度,在抗-CD3ε抗体、抗-CD28抗体和IL-12作用下,加入抑制性寡脱氧核苷酸或对照寡脱氧核苷酸培养72 h后,用ELISA检测上清IFN-γ和IL-4的分泌,用RT-PCR检测细胞T-bet mRNA的表达。结果:抑制性寡脱氧核苷酸能明显抑制CD4+T细胞IFN-γ的分泌,促进IL-4的分泌,同时还抑制T-bet mRNA的表达。结论:在促Th1细胞因子刺激下,抑制性寡脱氧核苷酸对小鼠体外CD4+T细胞的功能性分化有作用,既促进Th2分化,又抑制Th1分化。Abstract： Objective To exlpore the effect of suppressive oligodeoxynucleotides （ Sup ODN ） on the Th 1 differentiation of CD4^＋T splenetic lymphocytes in mice. Methods The splenetic lymphocytes of BALB/c mice were separated, and then CD4^＋ cells were purified with immune magnetic CD4^＋ microbeads （positive selection ）. The purification was examined by fluorescence-activated cell sorter. CD4^＋ cells, anti-CD3ε, anti-CD28, IL-12 and Sup ODN or control oligodeoxynucleotides （Con ODN ） were co-incubated for 72 h. IFN-γ and IL-4 in the supernatant were detected using enzyme-linked immunosorbent assay. The expression of T-bet mRNA in CD4^＋ cells was tested by reverse transcription polymerase chain reaction. Results Sup ODN could significantly inhibit the re- lease of INF-γ and increase IL-4 production respectively （P 〈0.01 ）. T-bet mRNA of CD4^＋ lymphocytes was remarkably inhibited by Sup ODN as well （ P〉0.01 ）. Conclusion In the presence of pro-Th1-cytokines, Sup ODN may affect the differentiation of CD4^＋ T lymphocytes in vitro. Sup ODN can promote CD4^＋ T cells to differentiate into Th2, and suppress them into Th1.

关 键 词：抑制性寡脱氧核苷酸 TH1 淋巴细胞 T-BET 

分 类 号：R392.1[医药卫生—免疫学]