青杨脊虎天牛对杨树皮挥发物的行为反应  

Behavioral responses of Xylotrechus rusticus L. to poplar bark volatiles

作　　者：程立超 迟德富[2] 谢兴[2] 王广利[2] 

机构地区：[1]黑河市森林病虫防治检疫站,黑龙江黑河164300 [2]东北林业大学林学院,哈尔滨150040

出　　处：《生态学杂志》2009年第1期45-50,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30671694);黑龙江省科技攻关资助项目(GB06B304)

摘　　要：采用水蒸气蒸馏法分别提取白城二号杨、小黑杨、俄罗斯杨、中东杨、小青杨、小青×黑杨、大青杨、斯大林杨、银中杨和小叶杨等10种杨树皮的挥发性物质,并采用气相色谱-质谱联用技术对其进行定性和定量分析。结果表明:邻羟基苯甲醛、亚油酸和11,14,17-二十碳三烯酸甲基酯是10种杨树皮挥发物的共有组分;不同树种之间挥发性醛类、酯类、烃类成分及相对含量差异较大;苯酚、樟脑、δ-杜松烯等是特异性化合物。通过"Y"型嗅觉仪比较分析了青杨脊虎天牛雌雄成虫分别对20、60、90、120、150和180μl10种杨树皮挥发物的行为反应。结果表明:雄成虫对120、150、180μl10种杨树皮挥发物的选择性差异显著(P<0.05),雌成虫对60、90、120μl10种杨树皮挥发物的选择性差异显著(P<0.05)。同时利用风洞技术测定了雌雄成虫对10种杨树皮200μl挥发物及20g木屑的行为反应。结果表明:雌成虫对10种木屑的选择性差异显著(P<0.05),对10种杨树皮挥发物无显著差异(P>0.05);雄成虫对挥发物的选择性差异显著(P<0.05),对木屑的选择性无显著差异(P>0.05)。在确定青杨脊虎天牛对不同杨树皮挥发物的选择性具有显著差异的基础上,讨论了对青杨脊虎天牛有活性作用的化学物质及杨树树种。The volatiles in the barks of ten poplar species, namely Populus × xiaozhuanica W. Y. Hsu et Liang cv. ＇ Baicheng-2＇ , P. simonii Cart. x P. nigra L. , P. russkii Jabl. , P. berolinensis Dipp. , P. pseudo-simonii Kitag. , P. pseudo-simonii Kitag. x P. nigra L. , P. ussuriensis Kom. , P. stalintz Jabl. , P. alba L. × P. berolinensis Dipp. , and P. simonii Cart. , were steam-distillated, and their qualitative and quantitative analyses were made with gas chre- matography-mass spectrometry （GC-MS）. Salicylaldehyde, linolein acid, and 11,14,17-eicosa- trienoic acid methyl ester were the common components in the volatiles. Different poplar species had greater differences in the components of their volatiles and in the relative contents of aldehyde, ester, and hydrocarbon. Phenol, camphor, and 8-cadinene were the specific components. The behavioral responses of male and female Xylotrechus rusticus L. to the volatiles at the doses of 20, 60, 90, 120, 150 and 180 μl were observed with Y-tube olfactometer, and it was indicated that male adults had significant differences in the choice rate to the volatiles at the doses of 120, 150, or 180μl（P 〈0. 05）, while female adults had the differences at the doses of 60, 90, or 120μl （P 〈0. 05）. The test of the behavioral responses of male and female X. rusticus to 200 μl volatiles of poplar barks and to 20 g scraps of different poplar species by using wind tunnel technique indicated that for the females, their choice rate to the scraps was significantly different （P 〈 0. 05 ）, but the choice rate to the volatiles had no significant difference （ P 〉 0. 05 ）. For the males, the choice rate to the volatiles was significantly different （ P 〈 0. 05 ） , while the choice rate to the scraps had lesser difference （P 〉 0. 05 ）. The potential active chemical substances and related poplar species were discussed, based on the significant differences in the choice rate of X. rusticus to the volatiles of different poplar barks.

关 键 词：青杨脊虎天牛 行为反应 杨树皮 挥发物 

分 类 号：S763.7[农业科学—森林保护学]