哮喘患儿淋巴细胞胞浆游离钙离子浓度变化及其与呼吸爆发功能的关系  

Cytosolic calcium concentration and its relevance to respiratory burst in lymphocytes from asthmatic children

作　　者：陆航[1] 王旭[1] 

机构地区：[1]浙江医科大学附属儿童医院

出　　处：《中国危重病急救医学》1998年第2期76-78,共3页Chinese Critical Care Medicine

基　　金：浙江省教委科研基金

摘　　要：目的：研究哮喘患儿淋巴细胞胞浆游离钙离子浓度变化及其与呼吸爆发功能的关系。方法：采用Ｑｕｉｎ２荧光技术直接测定淋巴细胞胞浆游离钙离子〔Ｃａ２＋〕ｉ和细胞色素Ｃ还原法测定淋巴细胞呼吸爆发Ｏ÷２释放量。结果：淋巴细胞胞浆〔Ｃａ２＋〕ｉ哮喘发作期明显高于稳定期（Ｐ＜０．０１），稳定期与正常儿童相近（Ｐ＞０．０５），内、外源型哮喘间无显著性差异；淋巴细胞呼吸爆发释放Ｏ÷２量哮喘发作期明显高于稳定期（Ｐ＜０．０１），稳定期仍高于正常儿童（Ｐ＜０．０５），内、外源型哮喘间无显著性差异；相关分析显示哮喘患儿淋巴细胞胞浆〔Ｃａ２＋〕ｉ与其呼吸爆发Ｏ÷２释放量呈正相关关系（ｒ＝０．４１，Ｐ＜０．０５）。结论：哮喘患儿淋巴细胞呼吸爆发功能增强，其Ｏ÷２释放量的增加在哮喘气道炎症中起重要作用，淋巴细胞胞浆〔Ｃａ２＋〕ｉ的增高与其活化有一定关系，但钙拮抗剂不宜作为哮喘治疗的主要药物。Objective:To investigate the cytosolic calcium concentration and its relevance to respiratory burst in lymphocytes from asthmatic children.Methods:The cytosolic calcium concentration 〔Ca2+〕i and superoxide generation in the peripheral lymphocytes were determined using Quin 2 as fluorescent indicator and the measurement of superoxide dismutaseinhibitable reduction of ferricytochrome C,respectively.Results:〔Ca2+〕i and O÷2 generation in lymphocytes from asthmatic children were significantly higher than those from healthy children.There were no significant differences in 〔Ca2+〕i and O÷2 generation in lymphocytes between intrinsic and extrinsic asthmatic children (P>005).〔Ca2+〕i in lymphocytes positively correlated with its O÷2 generation (r=041,P<005).Conclusions:The study indicates that easier activation of lymphocytes in asthma is partly due to the increment of 〔Ca2+〕i.The lymphocytes from asthmatic children produce more O÷2 when it is stimulated than those lymphocytes from healthy children,which may play an important role in the pathogenesis of asthma.

关 键 词：哮喘 淋巴细胞 胞浆 游离钙离子 呼吸爆发 儿童 

分 类 号：R725.622.5[医药卫生—儿科]