环氧乙烷的遗传毒性研究Ⅷ小鼠肝细胞DNA定量分析研究  

STUDY ON THE GENOTOXICITY OF ETHYLENE OXIDE  STUDY OF DNA QUANTITATIVE ANALYSIS IN THE MOUSE HEPATOCYTES

作　　者：王金茂[1,2] 张树人[1,2] 陆静芬[1,2] 钟坤[1,2] 张龄[1,2] 

机构地区：[1]浙江医科大学 [2]上海职工医学院

出　　处：《癌变．畸变．突变》1998年第1期11-14,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金

摘　　要：本研究应用ＩＢＡＳ显微数字图象分析系统，对不同剂量环氧乙烷（ｅｔｈｙｌｅｎｅｏｘｉｄｅ，ＥｔＯ）染毒小鼠的肝细胞核ＤＮＡ含量进行测定的结果显示：染毒小鼠肝细胞核ＤＮＡ的含量均较对照组增高。特别引起注意的是雄性染毒小鼠肝细胞核ＤＮＡ的含量明显高于雌性，其中雄性染毒组与对照组、雄性染毒组与雌性染毒组之间，肝细胞核ＤＮＡ含量的平均值比较，均有显著差异，经Ｋ—Ｓ检验结果Ｐ＜０．０１。据此可以提示：环氧乙烷不但可以引起实验小鼠肝细胞ＤＮＡ的复制，诱发肝细胞抵近恶性变的倾向，而且雄性小鼠肝细胞对ＥｔＯ的敏感性明显高于雌性。With the digital image analytic system,this study is to quantitate the DNA in hepatocytes nuclei of exposed mice with different doses of ethylene oxide.The results showed that the quantity of DNA in the hepatocytes nuclei of exposed groups is higher than that in the control group.It is worth noticing,the DNA quantity in male groups is obviously higher than that in the females.By comparing the male exposed groups with control group and the male exposed groups with female exposed groups,their DNA quantity in the nuclei of hepatocytes are both significant different.The K-S test P<0.01. The data suggested that EtO not only caused the duplication of DNA in the experimental mice hepatocytes but also induced the tendency of approching malignant changes in the hepatocytes.The hepatocytes in the male mice are more sensitive to EtO than those in the female.

关 键 词：环氧乙烷 肝细胞 DNA 定量分析 遗传毒性 

分 类 号：R994.3[医药卫生—毒理学] R992[医药卫生—药学]