同位素示踪SEC-ICP-MS研究大鼠体内镉的形态  

Study on CA speciation in different tissues of rats by stable isotope tracer with SEC-ICP-MS

作　　者：张建平[1] 庄峙厦[1,2] 陈成祥[1] 罗洁[1] 黄华斌[1] 齐士林[1] 王小如[1,2] 

机构地区：[1]厦门大学化学化工学院化学系,现代分析科学教育部重点实验室,厦门361005 [2]国家海洋局第一海洋研究所,青岛266061

出　　处：《分析试验室》2009年第1期21-25,共5页Chinese Journal of Analysis Laboratory

基　　金：福建省重大科技(2003Y005-04)和(2003Y003)项目资助

摘　　要：通过建立凝胶排阻色谱分离紫外与电感耦合等离子体质谱在线检测的联用技术,研究了灌喂低浓度镉富集同位素后大鼠各器官中镉与蛋白结合的形态。结果发现,镉主要在肝、肾、睾丸中与硫蛋白结合形成金属硫蛋白。与传统方法相比,该法危害性小、具有更接近慢性中毒、能将重金属来源分为天然来源和人为引入等特点。The experiment aims to study combination of Cd and proteins in different tissues of rat fed with Cd isotope by SEC-UV-ICP-MS. In testicle, liver and kidney, Cd-MTs were found to be the binding product of Cd and MTs. This method is much more accurate, humanl than traditional method, and be able to distinguish the heavy metal from nature and artificiality.

关 键 词：同位素示踪 SEC UV ICP-MS 金属硫蛋白 

分 类 号：O657.6[理学—分析化学]