电化学方法研究芦丁与鲱鱼精DNA之间的相互作用  

Study on interaction of fish sperm DNA with rutin by electrochemical methods

作　　者：王桂香[1] 马升勇[1] 王文鑫[1] 张丰[1] 

机构地区：[1]泰山学院材料与化学工程系,泰安271021

出　　处：《分析试验室》2009年第1期113-116,共4页Chinese Journal of Analysis Laboratory

基　　金：泰山学院引进人才(Y05-2-03)项目资助

摘　　要：采用循环伏安法和交流阻抗法研究了固定在羟基磷灰石薄膜上的DNA与芦丁之间的相互作用。在pH 5.6～7.0范围内,芦丁在DNA修饰电极上的峰电位随pH的增加向负方向移动;在50～800 mV/s扫描速度范围内电极过程同时受扩散和吸附控制,且扩散控制占主导作用;随溶液离子强度增大,芦丁在DNA修饰电极上的表观式量电位不断正移,表明芦丁与DNA之间存在一定的嵌入作用,二者结合形成了超分子化合物。The interactions of DNA immobilized on the hydroxyapatite （HAp） film with rutin were investigated by means of cyclic voltammetry （CV） and AC impedance. The results show that both the anodic and cathodic peak potentials shift negatively with increasing pH from 5.6 to 7.0. The redox peak currents for rutin at a DNA modified electrode have linear relationship with scan rate from 50 to 800 mV/s, the electrode process is controlled simultaneously by diffusion and adsorption, and the diffusion plays a main role. The apparent potential for rutin at the dsDNA/HAp- PVA/GCE shifts positively with increasing ionic strength of solution, indicating that the intercalation exists between rutin and DNA, leading to formation of supramolecular compounds.

关 键 词：DNA 羟基磷灰石 芦丁 玻碳电极 交流阻抗法 

分 类 号：O657.1[理学—分析化学]