川芎嗪甲酸对平滑肌细胞游离钙浓度的影响  

Effect of CTPZ on Cytosolic Free Calcium Concentration in Smooth Muscle Cells

作　　者：张立冬[1] 尹春萍[1] 邹慧[2] 刘妙娜[1] 林智成[1] 严书超[1] 刘继红[3] 

机构地区：[1]华中科技大学同济医学院同济医院,武汉430030 [2]四川出入境检验检疫局机场办 [3]华中科技大学同济医学院药学院

出　　处：《中国药师》2009年第1期9-11,共3页China Pharmacist

基　　金：湖北省科技攻关项目基金资助(项目编号:2007AA301B26-1)

摘　　要：目的:研究川芎嗪代谢产物川芎嗪甲酸(CTPZ)对大鼠阴茎海绵体平滑肌细胞(PCSMC)胞质内游离钙离子浓度的影响。方法:用新型Ca^(2+)荧光染色剂Fluo-3/AM负载大鼠PCSMC,细胞分为氯化钾(KCl)和去甲肾上腺素(NE)作用组,应用激光扫描共聚焦显微镜(LSCM)实时测定胞质内[Ca^(2+)]的变化,分别观察不同浓度的CTPZ对高钾和NE诱导胞质内钙浓度升高的影响,并与母药川芎嗪作用相比。结果:静息状态下,CTPZ对大鼠PCSMC胞质内[Ca^(2+)]无明显影响。1,10,100μmol·L^(-1)CTPZ能显著抑制高钾诱发的细胞内的钙浓度升高的影响,抑制率分别为(39.8±4.3)%,(49.2±3.6)%,(58.2±3.9)%。也能抑制1μmol·L^(-1)NE诱发钙库释放的细胞内[Ca^(2+)]升高,抑制率分别为(20.8±3.9)%,(32.3±2.5)%,(43.7±3.2)%。结论:CTPZ对大鼠PCSMC电压依赖性钙通道和细胞内钙库释放的抑制作用,能降低PCSMC胞质内[Ca^(2+)]水平,其作用效果比母药川芎嗪佳。To study the effects of CTPZ on intracellular free calcium [ Ca2 ~ ] i in cultured penis corpus cavernosum smooth muscle ceils （PCSMC） in Rat. Method ： The laser scanning confocal microscope （ LSCM ） was used, the [ Ca^2＋ ]i variations were investigated in cultured PCSMC loaded with Ca^2＋ indicator Fluo-3/AM. Compared with TMP, the effects of CTPZ was observed in different concentrations on [ Ca^2＋ ]i increase induced by high potassium and NE. Result： CTPZ had no obvious effect on resting PCSMC [ Ca^2＋ ]i,It was found that 1,10,100μmol· L-1 CTPZ significantly inhibited [ Ca^2＋]i increase induced by high potassium depolarization. The peak inhibition rates were （ 39.8 ＋ 4.3 ） %, （49.2 _＋ 3.6） % and （ 58.2 ＋ 3.9 ） % respectively. CTPZ could also inhibit cytosolic calcium pool release induced by 1 μmol·L - 1 NE. The peak inhibition rates were （20.8 ＋ 3.9） %, （32.3 ＋ 2.5 ） % and （43.7 ＋ 3.2） % respectively. Conclusion ： CTPZ can inhibit PCSMC [ Ca^2＋ ] i significantly by suppressing voltage dependent calcium channel and cytosolic calcium pool release in rat. Compared with TMP, its effect was better.

关 键 词：川芎嗪甲酸 平滑肌细胞 激光扫描共聚焦显微镜 钙通道 

分 类 号：R285.5[医药卫生—中药学]