HPLC法测定归芪补血口服液中黄芪甲苷的含量  

Determination the Content of Astagaloside in Guiqibuxue Oral Liquid by HPLC

作　　者：陈荣[1] 黄梦娴[1] 

机构地区：[1]广西医科大学制药厂,南宁530021

出　　处：《中国药师》2009年第1期77-79,共3页China Pharmacist

摘　　要：目的:建立归芪补血口服液中黄芪甲苷含量测定的HPLC方法,并将结果与薄层扫描(TLCS)法进行比较。方法:色谱柱为依利特C_(18)色谱柱(250mm×4.6mm,5μm),以乙腈:水(31:69)为流动相,检测波长为201nm。结果:黄芪甲苷的线性范围为0.5～15.7μg(r=0.999 8,n=5);平均加样回收率98.2%(RSD=0.73%,n=5)。HPLC法比TLCS法更简便、快速、准确。结论:本文所建立的HPLC法,准确、简便、快捷,可代替TLCS法用于制剂质量控制。To establish an HPLC method for determination the content of astragaloside in Guiqibuxue oral liquid. Method： The TLCS and HPLC was used to determine the content of astragaloside in Guiqibuxue oral liquid, Hypersil Elite C18 column（ 250 mm × 4.6mm,5μm） was used with acetonitrile-water （31 ： 69 ） as mobile phase, and the detection wavelength was at 201 nm. Result ： The recovery rate by HPLC was 98.2% and RSD was 0.73% （ n = 5 ）, the linear range was O. 5 - 15.7 μg （ r = 0. 999 8, n = 5 ）, and HPLC was more simple and accurate than TLCS. Conclusion ： The content of astragaloside in Guiqibuxue oral liquid can be determined by HPLC established in the article instead of TLCS.

关 键 词：高效液相色谱法 薄层扫描法 归芪补血口服液 黄芪甲苷 

分 类 号：R927.2[医药卫生—药学]