安宫牛黄丸对急性脑出血大鼠脑组织中一氧化氮合酶及单胺类神经递质的影响  

The Effect of Angong Bezoar Pill for Nitric Oxide Synthase and Monoamine Neurotransmitter in Brain Tissue of Rats' Brain Hemorrhage Acute Stage

作　　者：杨文清[1] 任玉录[2] 郭克锋[1] 朱银星[1] 

机构地区：[1]第四军医大学唐都医院,西安710038 [2]青海卫生职业技术学院,西宁810000

出　　处：《中国中医急症》2009年第1期83-84,共2页Journal of Emergency in Traditional Chinese Medicine

摘　　要：目的探讨安宫牛黄丸对大鼠脑出血急性期脑组织中一氧化氮合酶(NOS)及单胺类神经递质的影响。方法建立大鼠脑出血急性期模型,观察脑出血后4、24、72h脑组织中一氧化氮(NO)和NOS及海马和皮质区去甲肾上腺素(NE)、肾上腺素、多巴胺、5-羟色胺(5-HT)的含量及不同治疗方案下其含量的变化。结果模型动物损伤后4h脑组织中NO含量增加,NOS活性升高,与正常组相比有显著差异;72h达到高峰。安宫牛黄丸组72hNO含量和NOS活性明显低于模型组与小牛去蛋白血清组。脑出血后4h,模型组神经递质含量较正常组显著下降,以24h下降明显,72h又有回升并接近4h值;安宫牛黄丸组、小牛去蛋白血清组与模型组相比均有差异,但安宫牛黄丸组作用优于小牛去蛋白血清组。结论安宫牛黄丸对脑出血急性期的大脑具有保护作用。Objective： To explore the effects of Angong Bezoar Pill for nitric oxide synthase and monoamine neurotransmitter in brain tissue of acute stage of rats＇ brain hemorrhage. Methods：To found the model of rats＇ brain hemon＇hage acute stage. After brain hemorrhage 4, 24, 72h, the content and the change of nitrogen monoxidum, nitricoxide synthase in brain tissue and EA, DA, 5 - HT in seahorse and cerebral cortex were observed. Results： After injury 4h, the content of NO increased. The activity of NOS heightened,and 72h reached peak. The content of NO and the activity of NOS of Angong Bezoar Pill group degraded obviously, compared with serum of calf deproteinization and model group. After brain hemorrhage 4h, the content of monoamine neurotransmitter was degression, among the total 24h decreased most obviously. After 72h, the Angong Bezoar Pill group the content of monoamine neurotransmitter markedly heightened and the effect was better, compared with serum of calf deproteinization and model group. Conclusion：The Angong Bezoar Pill can protect brain tissue from rats＇brain hemorrhage acute stage.

关 键 词：脑出血 一氧化氮 一氧化氮合酶 单胺类神经递质 安宫牛黄丸 

分 类 号：R285.5[医药卫生—中药学]