中药熏蒸疗法对Ⅱ型胶原蛋白诱导型关节炎大鼠关节病理改变的影响  

Effect of the Chinese Herb Fumigation and Steaming Therapy on Pathology of Joint in CIA Rats

作　　者：吴名波[1] 沈鹰[1] 

机构地区：[1]广州军区广州总医院,广州510010

出　　处：《中国中医急症》2009年第1期96-98,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：广东省自然科学基金资助项目(No.06019700)

摘　　要：目的研究中药熏蒸疗法对Ⅱ型胶原蛋白诱导的关节炎(CIA)模型大鼠关节病理改变的影响。方法用小牛源Ⅱ型胶原复制CIA模型。清洁级雄性Wistar大鼠随机分为正常组、模型组、水熏组、低熏组、高熏组,正常组和模型组常规喂养,不作熏蒸处理;水熏组给予(57±1)℃蒸馏水熏蒸,蒸气温度为(39±1)℃;低熏组给予水熏组相同温度的3.33%中药液熏蒸;高熏组给予水熏组相同温度的6.67%中药液熏蒸,造模第41日处死大鼠,获取完整膝关节。光镜下观察炎性细胞浸润、纤维组织增生及滑膜细胞增生情况。结果中药熏蒸疗法对CIA大鼠骨关节组织的炎性细胞浸润、纤维组织增生及滑膜细胞增生有明显抑制作用。3种病理改变的总积分与模型组差异显著。结论中药熏蒸疗法治疗类风湿性关节炎的机制可能与抑制炎性细胞浸润、纤维组织增生及滑膜细胞增生有关。Objective： To study the effect to the Chinese herb fumigation and steaming therapy on pathology of joint in collagen 2 induced arthritis（CIA） rats. Method： The male Wistar rats were used to reproduet the CIA models by intradermal injecting CII,and divided into 5 groups randomly： normal group（no model） , model group （treated as normal group）, distilled water fumigation group（water fumigation group）, high dose fumigation group（the concentration of the Chinese herb liquor is 6.67% ）,low dose fumigation group（the concentration of the Chinese herb liquor is 3.33% ）. The temperature of all fumigation groups was （39 ± 1 ）℃. The pathological changes of each rat＇s joints were recorded 20d after model made. Result： The Chinese herb fumigation and steaming therapy could inhaibit inflammatory cell infiltration, fibroblast hyperplasia and synoviocyte hyperplasia in CIA rats. The total index had obvious difference with model group. Conclusion： The mechanism of curative effect of the Chinese herb fumigation and steaming therapy on rheumatoid arthritis concerns maybe inhibiting the inflammatory cell infiltration and fibroblast hyperplasia and synoviocyte hyperplasia.

关 键 词：类风湿性关节炎 中药熏蒸疗法 CIA 病理 大鼠 

分 类 号：R285.5[医药卫生—中药学]