长针科线虫2种传毒种类和3种非传毒种类分子鉴定和亲缘分析  

Molecular characteristics and phylogenetic relationships between three non-virus-vector longidorids and two virus-vector ones

作　　者：杜宇[1] 段禄华[1] 周剑[1] 和万忠[1] 曹云华[1] 李斌[1] 陈光勇[1] 寸东义[1] 

机构地区：[1]云南出入境检验检疫局,云南昆明650228

出　　处：《云南大学学报（自然科学版）》2009年第1期84-89,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金："十一五"国家科技支撑计划重大资助项目(2006BAK10B06);国家质量监督检验检疫局资助项目(2007IK256)

摘　　要：裂尾剑线虫Xiphinema diversicaudatum和逸去长针线虫Longidorus elongatus是植物病毒的重要传毒线虫，具有重要的经济意义，被我国列入进境植物检疫性有害生物名单．通过对上述2种检疫性线虫和中国云南不同地区3种长针科线虫共7个种群rDNA的内转录间隔区（ITS）进行PCR扩增和测序，分别获得1400～2100bpPCR产物片段，通过与Genbank上5种8种群的ITS区序列进行分析，结果发现裂尾剑线虫与包括逸去长针线虫等在内的其他4种线虫的同源性仅有55％，逸去长针线虫与3种长针科非传毒线虫的同源性仅为56％．同种不同种群间的rDNA—ITS的基因变异分别从0～10％不等．Xiphinema diversicaudatum and Longidorus elongatus are economically important plant -parasitic nematodes as virus vectors. Both of them are on the lists of quarantine pests related to the import plants in China. Molecular methods and genetic analyses based on the DNA sequences of nuclear ribosomal DNA ITS were con- ducted to determine the longidorids species and phylogenetic relationships within 6 taxa 15 populations, including X. diversicaudatum , X. brevicollum , X. insigne, L. elongatus , L. intermedius as well as Paralongidorus maximus. Seven DNA sequences varied from 1 400 bp to 2 100 bp for 7 targeted samples collected from Netherlands and Yunnan, China, respectively, both homology tree and phylogenetic tree were set up using MEGA4.0 software. Among them,X, diversicaudatum has 55% similarity with the other 4 studied Longidorids nematodes. Similarly, there is 56% genetic divergence between L. elongatus and 3 other studied Longidorids. The genetic variation of the studied nematodes had ranged from 10% among X. diversicaudatum to zero among L. elongatus.

关 键 词：长针科线虫 分子鉴定 亲缘分析 

分 类 号：Q959.17[生物学—动物学]