老年高血压病人红细胞变形能力与膜钙依赖中性蛋白酶活性的关系  

THE CORRELATION BETWEEN ERYTHROCYTE DEFORMABILITY AND THE ACTIVITY OF Ca 2+ DEPENDENT PROTEASE IN ELDERLY PATIENTS WITH HYPERTENSION

作　　者：刘成玉[1,2,3,4,5] 张健[1,2,3,4,5] 纪新强 郭建金[1,2,3,4,5] 王秀丽 谭润鸾 

机构地区：[1]青岛医学院诊断学教研室 [2]中国海监船第一大队卫生所 [3]青岛医学院预防医学教研室 [4]青岛市胶南市红石崖卫生院 [5]青岛医学院生物化学教研室

出　　处：《青岛医学院学报》1998年第1期21-23,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：①目的探讨老年高血压病人红细胞变形能力（ＥＤ）与红细胞膜钙依赖中性蛋白酶（Ｃａｌｐａｉｎ）活性的关系。②方法采用红细胞变形能力测定仪、分光光度计和凝胶电泳法分别检测了６８例老年高血压病人和６０例老年前期高血压病人及８０例健康人红细胞滤过指数（ＥＦＩ）、红细胞膜Ｃａｌｐａｉｎ和Ｃａ２＋－ＡＴＰ酶活性及红细胞膜收缩蛋白（ＳＰ）相对含量的变化。③结果老年高血压病人ＥＦＩ，Ｃａｌｐａｉｎ活性明显增高，Ｃａ２＋－ＡＴＰ酶活性和ＳＰ相对含量明显降低，与老年前期病人和健康对照组比较差异均有极显著性（ｔ＝３．１７７～５．２０９，Ｐ均＜０．００１）。老年前期高血压病人各指标变化较老年前期健康人亦明显（ｔ＝２．６２６～３．０８４，Ｐ均＜０．０１）。老年高血压病人ＥＦＩ与Ｃａｌｐａｉｎ活性呈正相关（ｒ＝０．６２１，Ｐ＜０．００１），与Ｃａ２＋－ＡＴＰ酶活性和ＳＰ相对含量呈负相关（ｒ＝－０．５７６，－０．４９６，Ｐ＜０．００１）；红细胞膜Ｃａ２＋－ＡＴＰ酶活性和ＳＰ含量与Ｃａｌｐａｉｎ活性呈负相关（ｒ＝－０．５１１，－０．５２９，Ｐ＜０．００１）。④结论老年高血压病人ＥＤ降低与红细胞膜Ｃａｌｐａｉｎ活性增高所致的膜蛋白溶解和破坏有?Objective To explore the correlation between the erythrocyte deformability(ED) and the activities of Ca 2+ dependent protease(calpain) of erythrocyte membrane in elderly patients with hypertension. Methods The erythrocyte filtration index(EFI), calpain and Ca 2+ ATPase activities, and the relative content of spectrin(SP) were determined respectively in 68 elderly patients, 60 pre elderly patients and 80 controls by using the eryhtrocyte deformability apparatus, spectrophotometry and gel electrophoretic analysis. Results The EFI and the activity of calpain were significantly higher, the activity of Ca 2+ ATPase and the relative contents of SP in erythrocyte membrane were significantly lower in elderly patients than those in pre elderly patients and controls( t=3.177～5.209,P <0.001).The EFI was positively correlated with calpain activity in elderly patients( r=0.621, P <0.001),negatively correlated with Ca 2+ ATPase activity and SP relative content( r=-0. 576, -0. 496, P < 0. 001) . The erythrocyte membrane Ca 2+ ATPase activity and SP content was negatively correlated with the calpain activity( r=-0.511,-0.529,P <0.001). Conclusion The decrase of ED was closely related to the lysis and degradation of erythrocyte membrane skeleton proteins caused by the increase of calpain activity in elderly hypertensive patients.

关 键 词：高血压 红细胞变形能力 红细胞膜 CALPAIN 

分 类 号：R544.1[医药卫生—心血管疾病]