蒙古冰草Lea3抗旱基因片段的分离与鉴定  

Isolation and Characterization of a Lea3 Gene Fragment from Agropyron mongolicum Keng

作　　者：赵彦[1,2] 云锦凤[1] 石凤敏[1] 高翠萍[1] 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010018 [2]内蒙古农牧业科学院,内蒙古呼和浩特010031

出　　处：《华北农学报》2008年第6期64-67,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金资助项目(30760159);国家“973”项目子课题(2007CB108901-1)

摘　　要：研究旨在利用同源序列法分离蒙古冰草抗旱基因同源片段。根据已知禾本科植物抗旱基因Lea第3组的保守区段设计一对简并引物,采用PCR方法对蒙古冰草幼苗的基因组DNA进行扩增。获得了1个蒙古冰草的抗旱基因Lea3基因片段,长度为497 bp,包含124 bp的非翻译区,共编码124个氨基酸。核苷酸序列分析表明,该序列与小麦抗旱基因LEA第3组的Wrab19同源性为93%,与小麦受ABA诱导的WRAB1基因同源性为93%;与一个来源于玉米mRNA的逆境胁迫基因克隆9124同源性为96%;与大麦受ABA诱导的pHVA1基因同源性为94%;与大麦HVA1基因同源性为91%。Southern杂交表明该基因在蒙古冰草基因组中以基因家族形式存在。In this study, drought resistance gene homology fragment from Agropyron mongolicum Keng was isolated using homology-based method. A pair of degeneracy primers was designed according to the amino acid conserved regions of the cloned Gramineae plant group 3 Lea genes, One drought resistance gene has been obtained, by the polymerase chain reaction（PCR）using the genomic DNA of A. mongolicum Keng as template. It was named as Lea3. The nucleotide sequences was 497 bp, including 124 bp untranslated region, and encoded 124 amino acids. Homology research showed that the nucleotides of Lea3 was 93% identical to wheat group 3 Lea gene Wrab19,93% identical to wheat gene WRAB1, 96% identical to the clone 9124 from the mRNA of maize,94% identical to barley gene pHVA1 ,and 91% identical to barley gene HVA1. the analysis of its secondary structure and hydrophilicity indicated that 2-helixes were predominant feature of secondary structure, and this gene had high hydrophilic property. The result of Southern blot analysis revealed the existence of an Lea3 gene family in the A. mongolicum Keng genome.

关 键 词：蒙古冰草 LEA基因 同源序列 

分 类 号：S543[农业科学—作物学]