利用AFLP分子标记鉴定苹果砧木  

Identification of Apple Rootstocks with AFLP Molecular Markers

作　　者：韩蕾[1] 徐继忠[1] 李振侠[2] 孙叶红[1] 张媛[1] 邵建柱[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北科技大学生物科学与工程学院生物科学系,河北石家庄050018

出　　处：《华北农学报》2008年第6期171-175,共5页Acta Agriculturae Boreali-Sinica

基　　金：河北省科技厅资助项目(04220111D)

摘　　要：利用AFLP分子标记技术,对30个苹果砧木进行研究。从64对引物组合中筛选出3对多态性高、分辨能力强的引物用于扩增,共获得199条带,其中多态性带176条,多态性率为88.4%。扩增结果显示,3对引物组合在12个砧木中扩增出特征带,且每对引物组合均能将所有砧木鉴别开,表明AFLP技术用于苹果砧木鉴定的效率很高。通过聚类,对供试砧木的遗传关系进行分析,为中国优良苹果砧木资源的进一步收集和利用提供科学依据。Thirty apple rootstocks were subjected to AFLP analysis. Three primer combinations with high polymorphism and powerful distinctiveness selected from 64 primer combinations produced 199 fragments, of which 176 were polymorphic with a polymorphism percentage of 88.4% .The results showed that characteristic bands were amplified from 12 rootstocks, and any of the three primer combinations could identify all the rootstocks. AFLP was proved to be a very reliable method to identify apple rootstocks. The genetic relationship of the apple rootstocks was analyzed by clustered analysis, which provided the scientific basis for further collection and the use of the Chinese fine rootstock resources.

关 键 词：苹果砧木 鉴定 AFLP 

分 类 号：S661.01[农业科学—果树学]