牛分支杆菌ESAT-6基因的克隆与序列分析  

Cloning and Sequencing of ESAT-6 Gene From Bovine Mycobacterium

作　　者：李海涛[1,2] 刘艳环[2] 李艳[2] 王志刚[2] 苗利光[2] 

机构地区：[1]江苏科技大学生物与环境工程学院,江苏镇江212018 [2]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2008年第4期10-13,共4页Special Wild Economic Animal and Plant Research

摘　　要：从患结核病鹿的肺组织中粗分离牛分支杆菌制备DNA模板,用PCR对牛分支杆菌ESAT-6基因进行了扩增,将扩增的产物连接于测序载体pGEM-TE上克隆,经测序确定无误;并使用分子生物学软件对ESAT-6基因进行了分析。结果显示:ESAT-6基因编码的蛋白有较好的抗原性;ESAT-6基因在结核分支杆菌和牛分支杆菌各基因型中具有高度保守性。本研究为下—步研制重组标记疫苗奠定了基础。The gene of ESAT-6 from genome of bovine mycobacterium tuberculosis strain that from cervine tuberculosis was amplified by polymerase chain reaction （PCR） and inserted into pGEM-TE vector. After sequencing, we analyzed the gene encoding ESAT-6 protein with molecular biologic software. It suggested that ESAT-6 protein had high antigenicity. The conservatism of ESAT-6 gene was very high between mycobacterium tuberculosis and mycobacterium bovine. This work would establish the platform for the study of recombinant marker vaccine.

关 键 词：牛分支杆菌 ESAT-6基因 克隆 序列分析 

分 类 号：S852.618[农业科学—基础兽医学] Q785[农业科学—兽医学]