灰飞虱体内共生菌Wolbachia的groEL基因克隆及序列分析  

Cloning and Sequencing of groEL From Wolbachia Endosymbiont in Laodelphax striatellus Fallén

作　　者：季英华[1] 史文琦[1] 程兆榜[1] 周彤[1] 林玲[1] 范永坚[1] 周益军[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014

出　　处：《南京师大学报（自然科学版）》2008年第4期119-123,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省重大科技攻关(BE2005301);国家支撑计划(2006BAD02A16;2006BAD08A04);江苏省自然科学基金(BK2006164);农业部行业专项(nyzx07-051);国家自然科学基金(30300230)资助项目

摘　　要：利用特异性引物,首次在国内克隆获得灰飞虱(Laodelphax striatellusFalln)体内共生菌Wolbachia的groEL基因全长序列,其开放阅读框为1659bp,可编码552个氨基酸,GenBank登录号为:EF468716,与已发布的韩国分离物核苷酸一致性达99.9%,为进一步研究其与灰飞虱传播水稻条纹病毒的关系奠定了基础.GroEL gene from Wolbachia endosymbiont in small brown plant-hopper（ Laodelphax striatellus Fallen）, was amplified by PCR and cloned. The completed nucleotide sequence of the gene was determined, which was the first report in China. It has 1 659 bp nucleotides and encodes 552 amino acids. GenBank accession number is EF 468716. Comparison showed that this gene had 99. 9% identities on nucleotides level to DQ 356890, an isolate from South Korea. Based on this sequence, Further researches on function of groEL, such as its relation to rice stripe virus transmission, can be carried out.

关 键 词：灰飞虱 共生菌 WOLBACHIA 序列分析 GROEL 

分 类 号：S435[农业科学—农业昆虫与害虫防治]