正相高效液相色谱法测定梨中5种菊酯类农药多残留  

Determination of Five Pyrethroids in Pear by Normal-phase High Performance Liquid Chromatography

作　　者：史亚俊[1] 魏朝俊[2] 贾临芳[2] 王春娜[2] 张婧[1] 赵建庄[2] 

机构地区：[1]北京农学院植物科学技术系,北京102206 [2]北京农学院基础教学部,北京102206

出　　处：《北京农学院学报》2008年第4期25-28,共4页Journal of Beijing University of Agriculture

基　　金：北京市自然科学基金项目(项目编号:6052004);北京市教委项目(项目编号:KM200610020010)

摘　　要：建立正相高效液相色谱方法同时测定梨样品中氯菊酯、甲氰菊酯、氯氰菊酯、溴氰菊酯和高效氯氟氰菊酯5种菊酯类农药残留。采用混合溶剂正己烷-乙酸乙酯（80／20，V／V）提取，在优化的流动相溶剂正己烷-乙酸乙酯（95／5，V／V）及流速1mL／min，检测波长242nm条件下，用配备Zorbax Rx-Sil柱、紫外检测器的高效液相色谱仪进行待测组分的分离和测定，检出限范围在0．108～0．193ng／μL。添加回收率试验结果表明，梨样品中上述5种菊酯类农药加标回收率为74．0％～108．0％，相对标准偏差（RSD％，n=5）为2．4～7．4，其准确度和精密度均达到了农药残留分析的要求。A method for determination of permethrin, fenpropathrin, cypermethrin, deltamethrin and lambda-cyhalothrin residues in pear was proposed by normal-phase high performance liquid chromatography. The pyrethroids in pear sample were extracted by a mixture of n-hexane and ethyl acetate（80/20, V/V）. The HPLC operating conditions were Zorbax Rx-Sil column, n-hexane and ethyl acetate （95/5, V/V） as mobile phase with the flow rate of 1 mL/min and UV detection wavelength at 242 nm. The pyrethroids were analyzed by HPLC with UV detector under optimum conditions and quantified by external standard calibration method. The lowest detection limit of the method was 0. 108-0. 193 ng/μL. The average recoveries of the five pyrethroids ranged or 74.0%-108.0%, and the relative standard deviation （n=5） were 2.4%-7.4%.

关 键 词：菊酯类农药 正相高效液相色谱法 梨 

分 类 号：S481[农业科学—农药学] O657.7[农业科学—植物保护]